Elastase-1 Antibody (4H5): sc-517432

Elastase-1 Antibody (4H5) is a mouse monoclonal IgG2a kappa light chain antibody that detects the Elastase-1 protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). Anti-Elastase-1 antibody (4H5) is available as a non-conjugated format. Elastase-1 is a serine protease that belongs to the elastase subfamily of the peptidase S1 family, primarily secreted by the exocrine pancreas in mammals. However, in humans, Elastase-1 is transcriptionally silent due to mutations that inactivate its promoter and enhancer, which is a significant aspect of its regulation. Elastase-1 plays a crucial role in the digestion of elastin, fibrin, hemoglobin, and albumin, with its activity being inhibited by elafin. Notably, Elastase-1 is expressed exclusively in skin keratinocytes, where Elastase-1 localizes to the basal cell layer of the epidermis, potentially facilitating the detachment of cells from the basement membrane. In keratinocytes, Elastase-1 activity may be modulated by secretory leukocyte protease inhibitor (SLPI) rather than elafin. Furthermore, Elastase-1 has been implicated as a candidate for diffuse nonepidermolytic palmoplantar keratoderma (NEPPK), an autosomal dominant skin condition, highlighting its relevance in dermatological research and potential therapeutic targets.

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Elastase-1 Antibody (4H5) References:

1. Human elastase 1: evidence for expression in the skin and the identification of a frequent frameshift polymorphism.  |  Talas, U., et al. 2000. J Invest Dermatol. 114: 165-70. PMID: 10620133
2. Inactivity of recombinant ELA2B provides a new example of evolutionary elastase silencing in humans.  |  Szepessy, E. and Sahin-Tóth, M. 2006. Pancreatology. 6: 117-22. PMID: 16327289
3. Genomic organization of the human homologue of the rat pancreatic elastase I gene.  |  Kawashima, I., et al. 1992. DNA Seq. 2: 303-12. PMID: 1633328
4. Potential for Screening for Pancreatic Exocrine Insufficiency Using the Fecal Elastase-1 Test.  |  Domínguez-Muñoz, JE., et al. 2017. Dig Dis Sci. 62: 1119-1130. PMID: 28315028
5. Characterization of a silent gene for human pancreatic elastase I: structure of the 5'-flanking region.  |  Tani, T., et al. 1987. J Biochem. 101: 591-9. PMID: 3648024
6. Evaluation of Fecal Pancreatic Elastase-1 as a Measure of Pancreatic Exocrine Function in Children with Pancreatitis.  |  Islam, F., et al. 2023. Mymensingh Med J. 32: 430-436. PMID: 37002754
7. Chymotrypsin-like Elastase-1 Mediates Progressive Emphysema in Alpha-1 Antitrypsin Deficiency.  |  Devine, AJ., et al. 2023. Chronic Obstr Pulm Dis. 10: 380-391. PMID: 37534975
8. Changes in fecal elastase-1 following initiation of CFTR modulator therapy in pediatric patients with cystic fibrosis.  |  Stephenson, KG., et al. 2023. J Cyst Fibros. 22: 996-1001. PMID: 37758535
9. Genetic linkage studies in non-epidermolytic palmoplantar keratoderma: evidence for heterogeneity.  |  Kelsell, DP., et al. 1995. Hum Mol Genet. 4: 1021-5. PMID: 7544664
10. Physical mapping of the human ELA1 gene between D12S361 and D12S347 on chromosome 12q13.  |  Davies, RL., et al. 1995. Genomics. 29: 766-8. PMID: 8575772
11. Expression of SKALP/elafin during wound healing in human skin.  |  van Bergen, BH., et al. 1996. Arch Dermatol Res. 288: 458-62. PMID: 8844125
12. Evolutionary silencing of the human elastase I gene (ELA1).  |  Rose, SD. and MacDonald, RJ. 1997. Hum Mol Genet. 6: 897-903. PMID: 9175736

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Elastase-1 Antibody (4H5) has 0 citations in a variety of scientific publications.

Primary antibodies like Anti-Elastase-1 Antibody (4H5) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Elastase-1 Antibody (4H5) Specifications

Alternate Product Name: Elastase-1

Product Clone Name: 4H5

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids:

Epitope Target: Elastase-1

Epitope Species: human

Product Reactivity/Detection: Elastase-1

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 100 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 0 citations in various scientific publications

Available Conjugates/Forms for Elastase-1 Antibody (4H5): N/A