eIF5B Antibody (D-9): sc-393564
- eIF5B Antibody (D-9) is a mouse monoclonal IgG1 κ eIF5B antibody, cited in 6 publications, provided at 200 µg/ml
- raised against amino acids 721-1020 mapping near the C-terminus of eIF5B of human origin
- eIF5B Antibody (D-9) is recommended for detection of eIF5B of mouse, rat and human origin by WB, IP, IF and ELISA; also reactive with additional species, including and equine, canine and porcine
- Anti-eIF5B Antibody (D-9) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for eIF5B Antibody (D-9) for WB applications. These reagents are now offered in bundles with eIF5B Antibody (D-9) (see ordering information below).
eIF5B Antibody (D-9) is a mouse monoclonal IgG1 kappa light chain antibody that detects eIF5B protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). anti-eIF5B antibody (D-9) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. The eukaryotic translation initiation factor 5B (eIF5B) plays a crucial role in the initiation of protein synthesis by facilitating the binding of formylmethionine-tRNA to the ribosome, a process essential for the formation of the 80S initiation complex. eIF5B, consisting of 1,120 amino acids, is predominantly located in the cytoplasm and is vital for the regulation of translation, which is a key step in gene expression. Proper functioning of eIF5B ensures accurate and efficient protein synthesis, impacting cellular function and overall organismal health. eIF5B interacts with Annexin V, an anticoagulant protein, in a calcium and phospholipid-dependent manner, highlighting involvement in complex cellular processes. Given conservation across all three kingdoms of life, eIF5B serves as a universal initiation factor, underscoring fundamental importance in biology.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
eIF5B Antibody (D-9) References:
- Engaging the ribosome: universal IFs of translation. | Roll-Mecak, A., et al. 2001. Trends Biochem Sci. 26: 705-9. PMID: 11738593
- Initiation factor eIF5B catalyzes second GTP-dependent step in eukaryotic translation initiation. | Lee, JH., et al. 2002. Proc Natl Acad Sci U S A. 99: 16689-94. PMID: 12471154
- Structural insights on the translation initiation complex: ghosts of a universal initiation complex. | Allen, GS. and Frank, J. 2007. Mol Microbiol. 63: 941-50. PMID: 17238926
- Position of eukaryotic initiation factor eIF5B on the 80S ribosome mapped by directed hydroxyl radical probing. | Unbehaun, A., et al. 2007. EMBO J. 26: 3109-23. PMID: 17568775
- Molecular genetic structure-function analysis of translation initiation factor eIF5B. | Shin, BS. and Dever, TE. 2007. Methods Enzymol. 429: 185-201. PMID: 17913624
- Functional equivalence of translation factor eIF5B from Candida albicans and Saccharomyces cerevisiae. | Jun, KO., et al. 2008. Mol Cells. 25: 172-7. PMID: 18414002
- Cleavage of eukaryotic initiation factor eIF5B by enterovirus 3C proteases. | de Breyne, S., et al. 2008. Virology. 378: 118-22. PMID: 18572216
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
eIF5B Antibody (D-9) | sc-393564 | 200 µg/ml | $322.00 | |||
eIF5B Antibody (D-9): m-IgG Fc BP-HRP Bundle | sc-527694 | 200 µg Ab; 10 µg BP | $361.00 | |||
eIF5B Antibody (D-9): m-IgG1 BP-HRP Bundle | sc-533067 | 200 µg Ab; 20 µg BP | $361.00 | |||
eIF5B Antibody (D-9) AC | sc-393564 AC | 500 µg/ml, 25% agarose | $424.00 | |||
eIF5B Antibody (D-9) HRP | sc-393564 HRP | 200 µg/ml | $322.00 | |||
eIF5B Antibody (D-9) FITC | sc-393564 FITC | 200 µg/ml | $336.00 | |||
eIF5B Antibody (D-9) PE | sc-393564 PE | 200 µg/ml | $349.00 | |||
eIF5B Antibody (D-9) Alexa Fluor® 488 | sc-393564 AF488 | 200 µg/ml | $364.00 | |||
eIF5B Antibody (D-9) Alexa Fluor® 546 | sc-393564 AF546 | 200 µg/ml | $364.00 | |||
eIF5B Antibody (D-9) Alexa Fluor® 594 | sc-393564 AF594 | 200 µg/ml | $364.00 | |||
eIF5B Antibody (D-9) Alexa Fluor® 647 | sc-393564 AF647 | 200 µg/ml | $364.00 | |||
eIF5B Antibody (D-9) Alexa Fluor® 680 | sc-393564 AF680 | 200 µg/ml | $364.00 | |||
eIF5B Antibody (D-9) Alexa Fluor® 790 | sc-393564 AF790 | 200 µg/ml | $364.00 |