eIF4G Antibody (C-11): sc-377288

eIF4G Antibody (C-11) is a mouse monoclonal IgM antibody that detects eIF4G in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). eIF4G plays a crucial role in the initiation of protein synthesis in eukaryotic cells by forming part of the eukaryotic initiation complex eIF4F, which is essential for ribosome binding to mRNA. This complex, composed of eIF4G, eIF4E, and eIF4A, facilitates the unwinding of mRNA secondary structures, thereby allowing for efficient translation. eIF4G serves as a scaffold that links eIF4E, which binds to the mRNA "cap," and eIF4A, an ATP-dependent RNA helicase, to the eIF3 complex, which is vital for the recruitment of the ribosome. Proper functioning of eIF4G is critical for regulating protein synthesis, and any dysregulation can lead to various diseases, including cancer. With an ability to interact with multiple initiation factors, eIF4G is a key player in the control of gene expression and cellular response to environmental signals, making anti-eIF4G antibody (C-11) an invaluable tool for researchers studying translational regulation and its implications in health and disease.

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eIF4G Antibody (C-11) References:

1. mTOR-dependent stimulation of the association of eIF4G and eIF3 by insulin.  |  Harris, TE., et al. 2006. EMBO J. 25: 1659-68. PMID: 16541103
2. Translation initiation factor eIF4G-1 binds to eIF3 through the eIF3e subunit.  |  LeFebvre, AK., et al. 2006. J Biol Chem. 281: 22917-32. PMID: 16766523
3. Depletion of eIF4G from yeast cells narrows the range of translational efficiencies genome-wide.  |  Park, EH., et al. 2011. BMC Genomics. 12: 68. PMID: 21269496
4. MNK2 inhibits eIF4G activation through a pathway involving serine-arginine-rich protein kinase in skeletal muscle.  |  Hu, SI., et al. 2012. Sci Signal. 5: ra14. PMID: 22337810
5. 3-substituted indazoles as configurationally locked 4EGI-1 mimetics and inhibitors of the eIF4E/eIF4G interaction.  |  Yefidoff-Freedman, R., et al. 2014. Chembiochem. 15: 595-611. PMID: 24458973
6. Two related trypanosomatid eIF4G homologues have functional differences compatible with distinct roles during translation initiation.  |  Moura, DM., et al. 2015. RNA Biol. 12: 305-19. PMID: 25826663
7. Plant growth and fertility requires functional interactions between specific PABP and eIF4G gene family members.  |  Gallie, DR. 2018. PLoS One. 13: e0191474. PMID: 29381712
8. Ksp1-dependent phosphorylation of eIF4G modulates post-transcriptional regulation of specific mRNAs under glucose deprivation conditions.  |  Chang, Y. and Huh, WK. 2018. Nucleic Acids Res. 46: 3047-3060. PMID: 29438499
9. Novel alleles of rice eIF4G generated by CRISPR/Cas9-targeted mutagenesis confer resistance to Rice tungro spherical virus.  |  Macovei, A., et al. 2018. Plant Biotechnol J. 16: 1918-1927. PMID: 29604159
10. Molecular dynamics simulations revealed topological frustration in the binding-wrapping process of eIF4G with eIF4E.  |  Gao, M. and Huang, Y. 2024. Phys Chem Chem Phys. 26: 2073-2081. PMID: 38131207

The preferred eIF4G antibody is eIF4G Antibody (A-10): sc-133155.