eIF4E2 Antibody (YB-18): sc-100731

eIF4E2 Antibody (YB-18) is a mouse monoclonal IgG1 kappa light chain antibody that detects eIF4E2 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). Anti-eIF4E2 antibody (YB-18) is available as a non-conjugated format. eIF4E2, also known as 4EHP, IF4e, 4E-LP, or EIF4EL3, plays a crucial role in protein synthesis regulation by binding to the 7-methylguanosine cap of mRNA, which is essential for mRNA stability and efficient translation. This 245 amino acid protein is ubiquitously expressed and belongs to the eukaryotic initiation factor 4E family, vital for early stages of translation initiation. eIF4E2 competes with eIF4E (member 1) for cap binding, and post-translational modifications, such as ubiquitination by ISG15, enhance eIF4E2′s affinity for mRNA cap. This competitive binding influences overall protein synthesis efficiency, impacting cellular responses to various stimuli, including stress and growth factors. eIF4E2′s role in translation initiation regulation makes this protein an important target for research in cancer and other diseases where protein synthesis is dysregulated.

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eIF4E2 Antibody (YB-18) References:

1. Human homologue of ariadne promotes the ubiquitylation of translation initiation factor 4E homologous protein, 4EHP.  |  Tan, NG., et al. 2003. FEBS Lett. 554: 501-4. PMID: 14623119
2. Characterizing the interaction of the mammalian eIF4E-related protein 4EHP with 4E-BP1.  |  Tee, AR., et al. 2004. FEBS Lett. 564: 58-62. PMID: 15094042
3. Characterization of mammalian eIF4E-family members.  |  Joshi, B., et al. 2004. Eur J Biochem. 271: 2189-203. PMID: 15153109
4. ISG15 modification of the eIF4E cognate 4EHP enhances cap structure-binding activity of 4EHP.  |  Okumura, F., et al. 2007. Genes Dev. 21: 255-60. PMID: 17289916
5. Structures of the human eIF4E homologous protein, h4EHP, in its m7GTP-bound and unliganded forms.  |  Rosettani, P., et al. 2007. J Mol Biol. 368: 691-705. PMID: 17368478
6. Weak binding affinity of human 4EHP for mRNA cap analogs.  |  Zuberek, J., et al. 2007. RNA. 13: 691-7. PMID: 17369309
7. MicroRNAs recruit eIF4E2 to repress translation of target mRNAs.  |  Chen, S. and Gao, G. 2017. Protein Cell. 8: 750-761. PMID: 28755203
8. The eIF4E2-Directed Hypoxic Cap-Dependent Translation Machinery Reveals Novel Therapeutic Potential for Cancer Treatment.  |  Melanson, G., et al. 2017. Oxid Med Cell Longev. 2017: 6098107. PMID: 29317983
9. High EIF4E2 expression is an independent prognostic risk factor for poor overall survival and recurrence-free survival in uveal melanoma.  |  Yang, B., et al. 2021. Exp Eye Res. 206: 108558. PMID: 33785306
10. METTL16 promotes translation and lung tumorigenesis by sequestering cytoplasmic eIF4E2.  |  Wang, F., et al. 2023. Cell Rep. 42: 112150. PMID: 36840945
11. Cloning and characterization of human eIF4E genes.  |  Gao, M., et al. 1998. J Biol Chem. 273: 4622-8. PMID: 9468520
12. Cloning and characterization of 4EHP, a novel mammalian eIF4E-related cap-binding protein.  |  Rom, E., et al. 1998. J Biol Chem. 273: 13104-9. PMID: 9582349

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-eIF4E2 Antibody (YB-18) has 5 citations in a variety of scientific publications.

Primary antibodies like Anti-eIF4E2 Antibody (YB-18) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

eIF4E2 Antibody (YB-18) Specifications

Alternate Product Name: eIF4E2

Product Clone Name: YB-18

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 1-246

Epitope Target: eIF4E2

Epitope Species: human

Product Reactivity/Detection: eIF4E2

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 100 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 5 citations in various scientific publications

Available Conjugates/Forms for eIF4E2 Antibody (YB-18): N/A