eIF4AIII Antibody (3F1): sc-33632

eIF4AIII Antibody (3F1) is a mouse monoclonal IgG1 antibody that detects eIF4AIII in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), and immunofluorescence (IF). eIF4AIII plays a crucial role in the regulation of gene expression by being a key component of the exon junction complex (EJC), which is essential for mRNA processing and surveillance. The EJC assembles at exon-exon junctions following splicing, ensuring that only properly processed mRNAs are translated, thus preventing the accumulation of defective transcripts. This function is vital for maintaining cellular homeostasis and preventing diseases associated with aberrant gene expression. eIF4AIII preferentially associates with spliced mRNA, distinguishing eIF4AIII from eIF4AI and eIF4AII, which are predominantly located in the cytoplasm. The nuclear localization of eIF4AIII underscores eIF4AIII′s importance in the post-transcriptional regulation of gene expression, making eIF4AIII a significant target for studies related to RNA metabolism and its implications in various biological processes and diseases.

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eIF4AIII Antibody (3F1) References:

1. Eukaryotic translation initiation factor 4AIII (eIF4AIII) is functionally distinct from eIF4AI and eIF4AII.  |  Li, Q., et al. 1999. Mol Cell Biol. 19: 7336-46. PMID: 10523622
2. A nuclear translation-like factor eIF4AIII is recruited to the mRNA during splicing and functions in nonsense-mediated decay.  |  Ferraiuolo, MA., et al. 2004. Proc Natl Acad Sci U S A. 101: 4118-23. PMID: 15024115
3. eIF4AIII binds spliced mRNA in the exon junction complex and is essential for nonsense-mediated decay.  |  Shibuya, T., et al. 2004. Nat Struct Mol Biol. 11: 346-51. PMID: 15034551
4. Mutational analysis of human eIF4AIII identifies regions necessary for exon junction complex formation and nonsense-mediated mRNA decay.  |  Shibuya, T., et al. 2006. RNA. 12: 360-74. PMID: 16495234
5. Dynamic behavior of Arabidopsis eIF4A-III, putative core protein of exon junction complex: fast relocation to nucleolus and splicing speckles under hypoxia.  |  Koroleva, OA., et al. 2009. Plant Cell. 21: 1592-606. PMID: 19435936
6. Human eIF4AIII interacts with an eIF4G-like partner, NOM1, revealing an evolutionarily conserved function outside the exon junction complex.  |  Alexandrov, A., et al. 2011. Genes Dev. 25: 1078-90. PMID: 21576267
7. Human CWC22 escorts the helicase eIF4AIII to spliceosomes and promotes exon junction complex assembly.  |  Barbosa, I., et al. 2012. Nat Struct Mol Biol. 19: 983-90. PMID: 22961380
8. eIF4AIII enhances translation of nuclear cap-binding complex-bound mRNAs by promoting disruption of secondary structures in 5'UTR.  |  Choe, J., et al. 2014. Proc Natl Acad Sci U S A. 111: E4577-86. PMID: 25313076
9. The eIF4AIII RNA helicase is a critical determinant of human cytomegalovirus replication.  |  Ziehr, B., et al. 2016. Virology. 489: 194-201. PMID: 26773380
10. lncRNA HOXC-AS1 promotes gastric cancer via binding eIF4AIII by activating Wnt/β-catenin signaling.  |  Zhou, C., et al. 2020. J Gene Med. 22: e3202. PMID: 32307743

The preferred eIF4AIII antibody is eIF4AIII Antibody (B-2): sc-365549.