eIF3γ Antibody (E-10): sc-271283
- eIF3γ Antibody (E-10) is a mouse monoclonal IgG1 κ eIF3γ antibody, cited in 1 publications, provided at 200 µg/ml
- raised against amino acids 51-352 mapping at the C-terminus of eIF3γ of human origin
- eIF3 gamma Antibody (E-10) is recommended for detection of eIF3γ of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-eIF3 gamma Antibody (E-10) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgGκ BP-HRP is the preferred secondary detection reagent for eIF3γ Antibody (E-10) for WB applications. This reagent is now offered in a bundle with eIF3γ Antibody (E-10) (see ordering information below). For additional m-IgGκ BP conjugates see our complete list of Mouse IgG Binding Proteins.
eIF3γ Antibody (E-10) is a mouse monoclonal IgG1 kappa light chain antibody that detects eIF3 gamma protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). anti-eIF3γ antibody (E-10) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. eIF3 gamma protein plays a crucial role in the initiation of protein synthesis in eukaryotic cells, functioning as part of the eukaryotic initiation factor 3 (eIF3) complex, which is the largest of the eIF complexes. This complex is essential for the assembly of the 40S ribosomal subunit, facilitating the recruitment of the initiator tRNA and mRNA, thereby promoting the formation of the pre-initiation complex. The structural integrity of eIF3 is vital, as eIF3 not only stabilizes the eIF2-GTP-Met-tRNAiMet complex but also aids in the dissociation of 80S ribosomes into their 40S and 60S subunits. Dysregulation and overexpression of eIF3 proteins have been implicated in various cancers, highlighting their potential role in tumorigenesis and making eIF3 proteins a significant target for cancer research.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
eIF3γ Antibody (E-10) References:
- Interactions of eukaryotic translation initiation factor 3 (eIF3) subunit NIP1/c with eIF1 and eIF5 promote preinitiation complex assembly and regulate start codon selection. | Valásek, L., et al. 2004. Mol Cell Biol. 24: 9437-55. PMID: 15485912
- eIF3: a connecTOR of S6K1 to the translation preinitiation complex. | Peterson, TR. and Sabatini, DM. 2005. Mol Cell. 20: 655-7. PMID: 16337588
- Translation initiation factor eIF4G-1 binds to eIF3 through the eIF3e subunit. | LeFebvre, AK., et al. 2006. J Biol Chem. 281: 22917-32. PMID: 16766523
- Initiation factor eIF3 and regulation of mRNA translation, cell growth, and cancer. | Dong, Z. and Zhang, JT. 2006. Crit Rev Oncol Hematol. 59: 169-80. PMID: 16829125
- eIF3: a versatile scaffold for translation initiation complexes. | Hinnebusch, AG. 2006. Trends Biochem Sci. 31: 553-62. PMID: 16920360
- Individual overexpression of five subunits of human translation initiation factor eIF3 promotes malignant transformation of immortal fibroblast cells. | Zhang, L., et al. 2007. J Biol Chem. 282: 5790-800. PMID: 17170115
- Reconstitution reveals the functional core of mammalian eIF3. | Masutani, M., et al. 2007. EMBO J. 26: 3373-83. PMID: 17581632
- Measles virus N protein inhibits host translation by binding to eIF3-p40. | Sato, H., et al. 2007. J Virol. 81: 11569-76. PMID: 17686838
- An oncogenic role for the phosphorylated h-subunit of human translation initiation factor eIF3. | Zhang, L., et al. 2008. J Biol Chem. 283: 24047-60. PMID: 18544531
- EIF3G is associated with narcolepsy across ethnicities. | Holm, A., et al. 2015. Eur J Hum Genet. 23: 1573-80. PMID: 25669430
- An interaction between eIF4A3 and eIF3g drives the internal initiation of translation. | Chang, J., et al. 2023. Nucleic Acids Res. 51: 10950-10969. PMID: 37811880
- In vitro reconstitution of SARS-CoV-2 Nsp1-induced mRNA cleavage reveals the key roles of the N-terminal domain of Nsp1 and the RRM domain of eIF3g. | Abaeva, IS., et al. 2023. Genes Dev. 37: 844-860. PMID: 37821106
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
eIF3γ Antibody (E-10) | sc-271283 | 200 µg/ml | $322.00 | |||
eIF3γ Antibody (E-10): m-IgGκ BP-HRP Bundle | sc-521901 | 200 µg Ab, 40 µg BP | $361.00 | |||
eIF3γ Antibody (E-10) AC | sc-271283 AC | 500 µg/ml, 25% agarose | $424.00 | |||
eIF3γ Antibody (E-10) HRP | sc-271283 HRP | 200 µg/ml | $322.00 | |||
eIF3γ Antibody (E-10) FITC | sc-271283 FITC | 200 µg/ml | $336.00 | |||
eIF3γ Antibody (E-10) PE | sc-271283 PE | 200 µg/ml | $349.00 | |||
eIF3γ Antibody (E-10) Alexa Fluor® 488 | sc-271283 AF488 | 200 µg/ml | $364.00 | |||
eIF3γ Antibody (E-10) Alexa Fluor® 546 | sc-271283 AF546 | 200 µg/ml | $364.00 | |||
eIF3γ Antibody (E-10) Alexa Fluor® 594 | sc-271283 AF594 | 200 µg/ml | $364.00 | |||
eIF3γ Antibody (E-10) Alexa Fluor® 647 | sc-271283 AF647 | 200 µg/ml | $364.00 | |||
eIF3γ Antibody (E-10) Alexa Fluor® 680 | sc-271283 AF680 | 200 µg/ml | $364.00 | |||
eIF3γ Antibody (E-10) Alexa Fluor® 790 | sc-271283 AF790 | 200 µg/ml | $364.00 |