eIF3α Antibody (H-1): sc-376651

eIF3α Antibody (H-1) is a mouse monoclonal IgM antibody that detects eIF3α protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). anti-eIF3α antibody (H-1) is available as the non-conjugated form. eIF3α protein plays a crucial role in the initiation of protein synthesis in eukaryotic cells, acting as a key component of the eukaryotic initiation factor 3 (eIF3) complex, which is the largest of the eIF complexes. This complex is essential for the assembly of the 40S ribosomal subunit, facilitating the recruitment of the initiator tRNA and mRNA, thereby promoting the formation of the pre-initiation complex. Proper functioning of eIF3α is vital for maintaining efficient protein synthesis, which is critical for cell growth and proliferation. Dysregulation of eIF3α has been implicated in various cancers, highlighting eIF3α′s potential as a biomarker for tumorigenesis and a target for therapeutic intervention. Using eIF3α (H-1) monoclonal antibody in research can provide valuable insights into protein synthesis mechanisms and their implications in disease states.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

eIF3α Antibody (H-1) References:

1. Interactions of eukaryotic translation initiation factor 3 (eIF3) subunit NIP1/c with eIF1 and eIF5 promote preinitiation complex assembly and regulate start codon selection.  |  Valásek, L., et al. 2004. Mol Cell Biol. 24: 9437-55. PMID: 15485912
2. eIF3: a connecTOR of S6K1 to the translation preinitiation complex.  |  Peterson, TR. and Sabatini, DM. 2005. Mol Cell. 20: 655-7. PMID: 16337588
3. Translation initiation factor eIF4G-1 binds to eIF3 through the eIF3e subunit.  |  LeFebvre, AK., et al. 2006. J Biol Chem. 281: 22917-32. PMID: 16766523
4. Initiation factor eIF3 and regulation of mRNA translation, cell growth, and cancer.  |  Dong, Z. and Zhang, JT. 2006. Crit Rev Oncol Hematol. 59: 169-80. PMID: 16829125
5. eIF3: a versatile scaffold for translation initiation complexes.  |  Hinnebusch, AG. 2006. Trends Biochem Sci. 31: 553-62. PMID: 16920360
6. Individual overexpression of five subunits of human translation initiation factor eIF3 promotes malignant transformation of immortal fibroblast cells.  |  Zhang, L., et al. 2007. J Biol Chem. 282: 5790-800. PMID: 17170115
7. Reconstitution reveals the functional core of mammalian eIF3.  |  Masutani, M., et al. 2007. EMBO J. 26: 3373-83. PMID: 17581632
8. Measles virus N protein inhibits host translation by binding to eIF3-p40.  |  Sato, H., et al. 2007. J Virol. 81: 11569-76. PMID: 17686838
9. An oncogenic role for the phosphorylated h-subunit of human translation initiation factor eIF3.  |  Zhang, L., et al. 2008. J Biol Chem. 283: 24047-60. PMID: 18544531

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-eIF3α Antibody (H-1) has 3 citations in a variety of scientific publications.

Primary antibodies like Anti-eIF3α Antibody (H-1) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

eIF3α Antibody (H-1) Specifications

Alternate Product Name: eIF3α

Product Clone Name: H-1

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgM kappa light chain

Amino Acids: 9-258

Epitope Target: eIF3α

Epitope Species: human

Product Reactivity/Detection: eIF3α

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgM kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 3 citations in various scientific publications

Available Conjugates/Forms for eIF3α Antibody (H-1): N/A