Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
eIF3η Double Nickase Plasmid (h): sc-400817-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- eIF3η Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- eIF3η Double Nickase Plasmid (h) and eIF3η Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting EIF3B. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: eIF3η Antibody (C-5): sc-137214
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
eIF3η Double Nickase Plasmid (h) | sc-400817-NIC | 20 µg | $410.00 | |||
eIF3η Double Nickase Plasmid (h2) | sc-400817-NIC-2 | 20 µg | $410.00 |
EIF3B encodes the eukaryotic translation initiation factor 3 subunit B (eIF3η), a core component of the multisubunit eIF3 complex that scaffolds initiation factors and the 40S ribosomal subunit to promote mRNA recruitment and start-codon recognition. Through its role in cap-dependent translation initiation, eIF3η contributes to proteostasis, cell-cycle progression, and adaptive translational control during cellular stress. Altered eIF3 complex function has been linked to dysregulated protein synthesis programs observed in proliferative and stress-response phenotypes, making EIF3B a useful node for studying translational reprogramming. EIF3B perturbation is therefore relevant to research on growth signaling and translation control mechanisms that are frequently remodeled in disease-associated cellular states.
eIF3η Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the EIF3B locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within EIF3B. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt EIF3B function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of EIF3B-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.