eIF2Bγ Antibody (F-7): sc-514230
- eIF2Bγ Antibody (F-7) is a mouse monoclonal IgG1 κ eIF2Bγ antibody provided at 200 µg/ml
- specific for an epitope mapping between amino acids 8-30 near the N-terminus of eIF2Bγ of human origin
- eIF2B gamma Antibody (F-7) is recommended for detection of eIF2Bγ of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-eIF2B gamma Antibody (F-7) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for eIF2Bγ Antibody (F-7) for WB applications. These reagents are now offered in bundles with eIF2Bγ Antibody (F-7) (see ordering information below).
eIF2Bγ Antibody (F-7) is a mouse monoclonal IgG1 kappa light chain antibody that detects eIF2B gamma protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). anti-eIF2Bγ antibody (F-7) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. eIF2B gamma protein plays a crucial role in regulating protein synthesis in eukaryotic cells, functioning as a key component of the eukaryotic initiation factor 2B (eIF2B) complex, which is essential for exchanging GDP for GTP on the eIF2 complex. This exchange is vital for forming the eukaryotic initiation complex, which is necessary for recruiting the ribosomal subunit to mRNA, thereby facilitating translation initiation. eIF2B complex is composed of five subunits: eIF2Bα, eIF2Bβ, eIF2Bγ, eIF2Bδ, and eIF2Bε, with eIF2Bε subunit exhibiting guanine nucleotide exchange factor activity, which is enhanced in the presence of other subunits. Notably, eIF2 phosphorylation can inhibit guanine nucleotide exchange factor activity of eIF2B, a process dependent on eIF2Bα subunit, highlighting intricate regulatory mechanisms that control protein synthesis in response to cellular stress.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
eIF2Bγ Antibody (F-7) References:
- Structure and sequence of the gene encoding the alpha-subunit of rat translation initiation factor-2B. | Flowers, KM., et al. 1995. Biochim Biophys Acta. 1264: 163-7. PMID: 7495858
- The delta-subunit of murine guanine nucleotide exchange factor eIF-2B. Characterization of cDNAs predicts isoforms differing at the amino-terminal end. | Henderson, RA., et al. 1994. J Biol Chem. 269: 30517-23. PMID: 7982969
- Cloning and characterization of cDNAs encoding the epsilon-subunit of eukaryotic initiation factor-2B from rabbit and human. | Asuru, AI., et al. 1996. Biochim Biophys Acta. 1307: 309-17. PMID: 8688466
- Cloning of cDNA for the gamma-subunit of mammalian translation initiation factor 2B, the guanine nucleotide-exchange factor for eukaryotic initiation factor 2. | Price, NT., et al. 1996. Biochem J. 318 (Pt 2): 631-6. PMID: 8809057
- eIF2B, the guanine nucleotide-exchange factor for eukaryotic initiation factor 2. Sequence conservation between the alpha, beta and delta subunits of eIF2B from mammals and yeast. | Price, NT., et al. 1996. Biochem J. 318 (Pt 2): 637-43. PMID: 8929216
- Subunit assembly and guanine nucleotide exchange activity of eukaryotic initiation factor-2B expressed in Sf9 cells. | Fabian, JR., et al. 1997. J Biol Chem. 272: 12359-65. PMID: 9139680
- Eukaryotic initiation factor 2B (eIF2B). | Webb, BL. and Proud, CG. 1997. Int J Biochem Cell Biol. 29: 1127-31. PMID: 9438375
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
eIF2Bγ Antibody (F-7) | sc-514230 | 200 µg/ml | $322.00 | |||
eIF2Bγ Antibody (F-7): m-IgG Fc BP-HRP Bundle | sc-527872 | 200 µg Ab; 10 µg BP | $361.00 | |||
eIF2Bγ Antibody (F-7): m-IgG1 BP-HRP Bundle | sc-533245 | 200 µg Ab; 20 µg BP | $361.00 | |||
eIF2Bγ Antibody (F-7) AC | sc-514230 AC | 500 µg/ml, 25% agarose | $424.00 | |||
eIF2Bγ Antibody (F-7) HRP | sc-514230 HRP | 200 µg/ml | $322.00 | |||
eIF2Bγ Antibody (F-7) FITC | sc-514230 FITC | 200 µg/ml | $336.00 | |||
eIF2Bγ Antibody (F-7) PE | sc-514230 PE | 200 µg/ml | $349.00 | |||
eIF2Bγ Antibody (F-7) Alexa Fluor® 488 | sc-514230 AF488 | 200 µg/ml | $364.00 | |||
eIF2Bγ Antibody (F-7) Alexa Fluor® 546 | sc-514230 AF546 | 200 µg/ml | $364.00 | |||
eIF2Bγ Antibody (F-7) Alexa Fluor® 594 | sc-514230 AF594 | 200 µg/ml | $364.00 | |||
eIF2Bγ Antibody (F-7) Alexa Fluor® 647 | sc-514230 AF647 | 200 µg/ml | $364.00 | |||
eIF2Bγ Antibody (F-7) Alexa Fluor® 680 | sc-514230 AF680 | 200 µg/ml | $364.00 | |||
eIF2Bγ Antibody (F-7) Alexa Fluor® 790 | sc-514230 AF790 | 200 µg/ml | $364.00 | |||
eIF2Bγ (F-7) Neutralizing Peptide | sc-514230 P | 100 µg/0.5 ml | $69.00 |