eIF2Bε Antibody (B-7): sc-55558
- eIF2Bε Antibody (B-7) is a mouse monoclonal IgG1 κ eIF2Bε antibody, cited in 7 publications, provided at 200 µg/ml
- raised against amino acids 422-711 mapping near the C-terminus of eIF2Bε of human origin
- eIF2B epsilon Antibody (B-7) is recommended for detection of eIF2Bε of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
- Anti-eIF2B epsilon Antibody (B-7) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for eIF2Bε Antibody (B-7) for WB and IHC(P) applications. These reagents are now offered in bundles with eIF2Bε Antibody (B-7) (see ordering information below).
eIF2Bε Antibody (B-7) is a mouse monoclonal IgG1 kappa light chain antibody that detects eIF2B epsilon protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). eIF2Bε Antibody (B-7) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. eIF2B epsilon protein plays a crucial role in regulating protein synthesis initiation in eukaryotic cells, functioning as a guanine nucleotide exchange factor that facilitates GDP for GTP exchange on eIF2 complex. This process is vital for forming eukaryotic initiation complex, which is essential for recruiting ribosomal subunit and subsequent translation of mRNA into proteins. eIF2B complex is composed of five subunits: eIF2Bα, eIF2Bβ, eIF2Bγ, eIF2Bδ, and eIF2Bε, with eIF2Bε exhibiting GEF activity that is significantly enhanced in presence of entire complex. Notably, eIF2 phosphorylation can inhibit GEF activity of eIF2B, a process dependent on eIF2Bα subunit, highlighting intricate regulatory mechanisms that control protein synthesis and potential implications for cellular stress responses.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
eIF2Bε Antibody (B-7) References:
- Structure and sequence of the gene encoding the alpha-subunit of rat translation initiation factor-2B. | Flowers, KM., et al. 1995. Biochim Biophys Acta. 1264: 163-7. PMID: 7495858
- The delta-subunit of murine guanine nucleotide exchange factor eIF-2B. Characterization of cDNAs predicts isoforms differing at the amino-terminal end. | Henderson, RA., et al. 1994. J Biol Chem. 269: 30517-23. PMID: 7982969
- Cloning and characterization of cDNAs encoding the epsilon-subunit of eukaryotic initiation factor-2B from rabbit and human. | Asuru, AI., et al. 1996. Biochim Biophys Acta. 1307: 309-17. PMID: 8688466
- Cloning of cDNA for the gamma-subunit of mammalian translation initiation factor 2B, the guanine nucleotide-exchange factor for eukaryotic initiation factor 2. | Price, NT., et al. 1996. Biochem J. 318 (Pt 2): 631-6. PMID: 8809057
- eIF2B, the guanine nucleotide-exchange factor for eukaryotic initiation factor 2. Sequence conservation between the alpha, beta and delta subunits of eIF2B from mammals and yeast. | Price, NT., et al. 1996. Biochem J. 318 (Pt 2): 637-43. PMID: 8929216
- Subunit assembly and guanine nucleotide exchange activity of eukaryotic initiation factor-2B expressed in Sf9 cells. | Fabian, JR., et al. 1997. J Biol Chem. 272: 12359-65. PMID: 9139680
- Eukaryotic initiation factor 2B (eIF2B). | Webb, BL. and Proud, CG. 1997. Int J Biochem Cell Biol. 29: 1127-31. PMID: 9438375
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
eIF2Bε Antibody (B-7) | sc-55558 | 200 µg/ml | $322.00 | |||
eIF2Bε Antibody (B-7): m-IgG Fc BP-HRP Bundle | sc-526864 | 200 µg Ab; 10 µg BP | $361.00 | |||
eIF2Bε Antibody (B-7): m-IgG1 BP-HRP Bundle | sc-532237 | 200 µg Ab; 20 µg BP | $361.00 | |||
eIF2Bε Antibody (B-7) AC | sc-55558 AC | 500 µg/ml, 25% agarose | $424.00 | |||
eIF2Bε Antibody (B-7) HRP | sc-55558 HRP | 200 µg/ml | $322.00 | |||
eIF2Bε Antibody (B-7) FITC | sc-55558 FITC | 200 µg/ml | $336.00 | |||
eIF2Bε Antibody (B-7) PE | sc-55558 PE | 200 µg/ml | $349.00 | |||
eIF2Bε Antibody (B-7) Alexa Fluor® 488 | sc-55558 AF488 | 200 µg/ml | $364.00 | |||
eIF2Bε Antibody (B-7) Alexa Fluor® 546 | sc-55558 AF546 | 200 µg/ml | $364.00 | |||
eIF2Bε Antibody (B-7) Alexa Fluor® 594 | sc-55558 AF594 | 200 µg/ml | $364.00 | |||
eIF2Bε Antibody (B-7) Alexa Fluor® 647 | sc-55558 AF647 | 200 µg/ml | $364.00 | |||
eIF2Bε Antibody (B-7) Alexa Fluor® 680 | sc-55558 AF680 | 200 µg/ml | $364.00 | |||
eIF2Bε Antibody (B-7) Alexa Fluor® 790 | sc-55558 AF790 | 200 µg/ml | $364.00 |