eIF2α Antibody (G-12): sc-133227
- eIF2α Antibody (G-12) is a mouse monoclonal IgG2b κ, cited in 23 publications, provided at 200 µg/ml
- raised against amino acids 1-315 representing full length eIF2α of human origin
- recommended for detection of eIF2α of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
- See eIF2α (D-3): sc-133132 for eIF2α antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
- m-IgG2b BP-HRP is the preferred secondary detection reagent for eIF2α Antibody (G-12) for WB and IHC(P) applications. This reagent is now offered in a bundle with eIF2α Antibody (G-12) (see ordering information below).
eIF2α Antibody (G-12) is a mouse monoclonal IgG2b kappa light chain antibody raised against amino acids 1-315, representing the full-length eIF2α of human origin. eIF2α (eukaryotic translation initiation factor 2 subunit alpha) is a pivotal component of the eukaryotic initiation complex that regulates the initiation of protein synthesis. Located predominantly in the cytoplasm, eIF2α binds GTP and initiator methionyl-tRNA to form a ternary complex, which associates with the 40S ribosomal subunit to facilitate the assembly of the 80S ribosome. This process is essential for the accurate translation of mRNA into proteins. The phosphorylation state of eIF2α is a critical regulatory point. Unphosphorylated eIF2α promotes normal protein synthesis, while phosphorylation at Ser51 in response to cellular stress inhibits GDP/GTP exchange, leading to a reduction in global protein synthesis. This regulatory mechanism allows cells to adapt to stress conditions such as nutrient deprivation, viral infection, and endoplasmic reticulum stress by conserving resources and preventing the accumulation of misfolded proteins. Dysregulation of eIF2α function is implicated in various diseases, including neurodegenerative disorders and cancer, underscoring its importance in maintaining cellular homeostasis. anti-eIF2α antibody (G-12) reacts with mouse, rat, and human eIF2α and is suitable for applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA).
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
eIF2α Antibody (G-12) | sc-133227 | 200 µg/ml | $322.00 | |||
eIF2α Antibody (G-12): m-IgG2b BP-HRP Bundle | sc-548488 | 200 µg Ab; 10 µg BP | $361.00 |