eIF2α CRISPR/CAS9 KO Plasmid (h): sc-400199. Western blot analysis of eIF2α expression in empty vector control (A) and eIF2α CRISPR/CAS9 KO Plasmid transfected (B) HEK293T whole cell lysates. Blot probed with eIF2α (D-3): sc-133132. β-actin (C4): sc-47778 used as specificity and loading control.
eIF2α CRISPR/Cas9 KO Plasmid (h): sc-400199
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- eIF2α CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the eIF2α genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: eIF2α Antibody (D-3): sc-133132
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
eIF2α CRISPR/Cas9 KO Plasmid (h) | sc-400199 | 20 µg | $397.00 |