EHZF CRISPR/Cas9 KO Plasmid (m): sc-432523
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- EHZF CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the EHZF genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
EHZF CRISPR/Cas9 KO Plasmid (m) | sc-432523 | 20 µg | $397.00 |
Overview
Zfp521 encodes the zinc-finger transcription factor EHZF, a nuclear regulator implicated in coordinating lineage commitment and developmental gene expression programs in mouse cells. EHZF modulates transcriptional networks involved in progenitor maintenance and differentiation, with reported crosstalk to pathways governing osteogenic, hematopoietic, and neural fate decisions. Through context-dependent interactions with chromatin-associated cofactors, Zfp521 influences epigenetic state, cell-cycle progression, and maturation of specialized cell types. Dysregulated Zfp521 activity has been associated in the literature with altered differentiation and proliferative phenotypes relevant to modeling developmental abnormalities and oncogenic transformation in experimental systems.
EHZF CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Zfp521 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Zfp521 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Zfp521 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish EHZF protein expression.
This CRISPR knockout system enables efficient generation of Zfp521-deficient cell models for investigation of EHZF signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Zfp521 exon(s) critical for EHZF function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Zfp521 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by EHZF CRISPR/Cas9 KO Plasmid (m) and EHZF CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Zfp521 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by EHZF HDR Plasmid (m) and EHZF HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Zfp521 homology arms to support homology-directed repair at defined Zfp521 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.