EHMT1 CRISPR/Cas9 KO Plasmid (h): sc-402460
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- EHMT1 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the EHMT1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
EHMT1 CRISPR/Cas9 KO Plasmid (h) | sc-402460 | 20 µg | $397.00 |
Overview
EHMT1 (euchromatic histone lysine methyltransferase 1) encodes a SET domain–containing methyltransferase that primarily catalyzes mono- and dimethylation of histone H3 lysine 9 (H3K9me1/2), promoting transcriptional repression within euchromatin. As part of epigenetic regulatory complexes with partners such as EHMT2/G9a, EHMT1 helps shape chromatin accessibility, coordinate lineage-specific gene expression programs, and stabilize cellular identity during development and differentiation. EHMT1-dependent H3K9 methylation interfaces with DNA methylation and heterochromatin organization to influence genome stability and long-range gene regulation. Dysregulation of EHMT1 activity or dosage has been linked to neurodevelopmental phenotypes and altered neuronal gene expression, and is studied in contexts including synaptic function, cognition-related pathways, and chromatin-associated disease mechanisms.
EHMT1 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the EHMT1 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the EHMT1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the EHMT1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish EHMT1 protein expression.
This CRISPR knockout system enables efficient generation of EHMT1-deficient cell models for investigation of EHMT1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting EHMT1 exon(s) critical for EHMT1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple EHMT1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by EHMT1 CRISPR/Cas9 KO Plasmid (h) and EHMT1 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the EHMT1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by EHMT1 HDR Plasmid (h) and EHMT1 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by EHMT1 homology arms to support homology-directed repair at defined EHMT1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.