EHHADH Antibody (D-2): sc-393123

EHHADH Antibody (D-2) is a mouse monoclonal IgG1 kappa light chain antibody that detects EHHADH protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). anti-EHHADH antibody (D-2) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. EHHADH protein, also known as enoyl-CoA-hydratase:3-hydroxyacyl-CoA dehydrogenase, plays a critical role in the β-oxidation of fatty acids within peroxisomes, which are essential organelles for lipid metabolism. Specifically, EHHADH is involved in the L-hydroxy-specific classical β-oxidation pathway, which is crucial for the breakdown of very long straight-chain fatty acids. A deficiency in EHHADH can lead to the accumulation of fatty acids in the serum, resulting in severe neurological impairments and early mortality. EHHADH consists of 723 amino acids and features a vital tripeptide sequence at its carboxyl-terminus that is necessary for transport to peroxisomes. Interestingly, studies on EHHADH-null mice have shown that while there is a diminished response to peroxisome proliferators, there are no significant alterations in lipid metabolism, indicating that enoyl-CoAs may be redirected to the D-hydroxy-specific β-oxidation pathway for processing.

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EHHADH Antibody (D-2) References:

1. Absence of spontaneous peroxisome proliferation in enoyl-CoA Hydratase/L-3-hydroxyacyl-CoA dehydrogenase-deficient mouse liver. Further support for the role of fatty acyl CoA oxidase in PPARalpha ligand metabolism.  |  Qi, C., et al. 1999. J Biol Chem. 274: 15775-80. PMID: 10336479
2. A genome scan for renal function among hypertensives: the HyperGEN study.  |  DeWan, AT., et al. 2001. Am J Hum Genet. 68: 136-144. PMID: 11115379
3. Fatty acid metabolism pathway play an important role in carcinogenesis of human colorectal cancers by Microarray-Bioinformatics analysis.  |  Yeh, CS., et al. 2006. Cancer Lett. 233: 297-308. PMID: 15885896
4. Import of human bifunctional enzyme into peroxisomes of human hepatoma cells in vitro.  |  Chen, GL., et al. 1991. Biochem Biophys Res Commun. 178: 1084-91. PMID: 1651711
5. Peroxisomal disorders: the single peroxisomal enzyme deficiencies.  |  Wanders, RJ. and Waterham, HR. 2006. Biochim Biophys Acta. 1763: 1707-20. PMID: 17055078
6. Underlying mechanisms of pharmacology and toxicity of a novel PPAR agonist revealed using rodent and canine hepatocytes.  |  Guo, Y., et al. 2007. Toxicol Sci. 96: 294-309. PMID: 17255113
7. Identifying novel peroxisomal proteins.  |  Hawkins, J., et al. 2007. Proteins. 69: 606-16. PMID: 17636571
8. cDNA cloning of the human peroxisomal enoyl-CoA hydratase: 3-hydroxyacyl-CoA dehydrogenase bifunctional enzyme and localization to chromosome 3q26.3-3q28: a free left Alu Arm is inserted in the 3' noncoding region.  |  Hoefler, G., et al. 1994. Genomics. 19: 60-7. PMID: 8188243

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-EHHADH Antibody (D-2) has 7 citations in a variety of scientific publications.

Primary antibodies like Anti-EHHADH Antibody (D-2) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

EHHADH Antibody (D-2) Specifications

Alternate Product Name: EHHADH

Product Clone Name: D-2

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 424-723

Epitope Target: EHHADH

Epitope Species: human

Product Reactivity/Detection: EHHADH

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 7 citations in various scientific publications

Available Conjugates/Forms for EHHADH Antibody (D-2): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin