Date published: 2025-9-8

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EGFR 항체 (A-10): sc-373746

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데이터 시트
  • EGFR 항체 A-10 는 마우스 monoclonal IgG2a κ EGFR 항체, 238간행물에 인용, 이며 200 µg/ml으로 제공합니다.
  • human의 EGFR의 C-terminal 세포질 도메인 내의 아미노산 1114-1147 사이의 에피토프 매핑에 특이적임
  • EGFR 항체 (A-10)는 WB, IP, IF, IHC(P) and ELISA으로 mouse, rat and human유래의 EGFR 를 감지하는 데에 추천한다.
  • 항-EGFR 항체(A-10)는 IP용 agarose와 결합되어 이용 가능하며, WB, IHC(P), ELISA용 HRP와 결합되어 이용 가능하며, IF, IHC(P), FCM용 phycoerythrin 또는 FITC와 결합되어 이용 가능합니다.
  • WB (RGB), IF, IHC(P) 와FCM, RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647결합제품도 있습니다.
  • WB (NIR), IF와FCM,Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 680 or Alexa Fluor® 790 결합제품도 있습니다.
  • EGFR (A-10): sc-373746무료10 µg 샘플을 신청하려면 기술지원팀 (또는 로컬 디스트리뷰터)에 연락주세요.
  • m-IgG Fc BP-HRP, m-IgG2a BP-HRPm-IgGκ BP-HRP 는 EGFR 항체 (A-10) 가 WB and IHC(P) 에 응용될 때 우선으로 사용하는 이차 항체 검사 시약입니다.이 시약은EGFR 항체(A-10) 와 세트로 제공드립니다. (아래의 주문 정보를 참고해 주시길 바랍니다).

빠른 링크

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EGFR 항체(A-10)는 마우스, 쥐 및 인간 유래의 EGFR 단백질을 WB, IP, IF, IHC(P) 및 ELISA로 검출하는 IgG2a κ 마우스 단일 클론 EGFR 항체(HER1 항체 또는 ERBB1 항체로도 지정됨)입니다. EGFR 항체(A-10)는 비접합 항-EGFR 항체 형태와 아가로스, HRP, PE, FITC 및 여러 Alexa Fluor® 접합체를 포함한 여러 접합 형태의 항-EGFR 항체 모두로 사용할 수 있습니다. EGF 수용체 계열은 다양한 암종에서 자주 과발현되는 여러 관련 수용체 티로신 키나아제로 구성됩니다. 이 수용체 계열의 구성원에는 리간드 결합 시 호모다이머 또는 헤테로다이머를 형성하는 EGFR(HER1), Neu(ErbB-2, HER2), ErbB-3(HER3) 및 ErbB-4(HER4)가 포함됩니다. EGFR 유전자 산물의 엑손은 종종 삭제되거나 복제되어 각각 다양한 분자량으로 검출되는 결실 돌연변이(DM) 또는 텐덤 복제 돌연변이(TDM)를 생성합니다. EGFR은 표피 성장 인자(EGF), 형질 전환 성장 인자 α(TGFα), 암피레귤린 및 헤파린 결합 EGF(HB-EGF)를 포함한 여러 리간드와 결합합니다. 리간드 결합은 클라트린으로 코팅된 구덩이를 통해 EGFR의 내재화를 촉진하고 고유한 티로신 키나아제에 반응하여 이후 분해를 촉진합니다. EGFR은 장기 형태 형성과 조직의 유지 및 복구에 관여하지만, EGFR의 상향 조절은 종양 진행과 관련이 있습니다. EGFR의 발암 효과에는 DNA 합성 시작, 세포 성장 촉진, 침입 및 전이가 포함됩니다. EGFR을 제거하면 암세포의 세포 주기 정지, 세포 자멸사 또는 탈분화가 일어나며, 이는 EGFR이 효과적인 치료 표적이 될 수 있음을 시사합니다.

연구용으로만 사용가능합니다. 진단이나 치료용으로 사용불가합니다.

Alexa Fluor®는 미국 오리건주 Molecular Probes Inc.의 상표입니다.

LI-COR®와 Odyssey®는 LI-COR Biosciences의 등록 상표입니다.

주문정보

제품명카탈로그 번호 단위가격수량관심품목

EGFR 항체 (A-10)

sc-373746
200 µg/ml
$316.00

EGFR (A-10): m-IgG Fc BP-HRP 번들

sc-529548
200 µg Ab; 10 µg BP
$354.00

EGFR (A-10): m-IgGκ BP-HRP 번들

sc-522480
200 µg Ab, 40 µg BP
$354.00

EGFR (A-10): m-IgG2a BP-HRP 번들

sc-547328
200 µg Ab; 10 µg BP
$354.00

EGFR 항체 (A-10) AC

sc-373746 AC
500 µg/ml, 25% agarose
$416.00

EGFR 항체 (A-10) HRP

sc-373746 HRP
200 µg/ml
$316.00

EGFR 항체 (A-10) FITC

sc-373746 FITC
200 µg/ml
$330.00

EGFR 항체 (A-10) PE

sc-373746 PE
200 µg/ml
$343.00

EGFR 항체 (A-10) Alexa Fluor® 488

sc-373746 AF488
200 µg/ml
$357.00

EGFR 항체 (A-10) Alexa Fluor® 546

sc-373746 AF546
200 µg/ml
$357.00

EGFR 항체 (A-10) Alexa Fluor® 594

sc-373746 AF594
200 µg/ml
$357.00

EGFR 항체 (A-10) Alexa Fluor® 647

sc-373746 AF647
200 µg/ml
$357.00

EGFR 항체 (A-10) Alexa Fluor® 680

sc-373746 AF680
200 µg/ml
$357.00

EGFR 항체 (A-10) Alexa Fluor® 790

sc-373746 AF790
200 µg/ml
$357.00

EGFR (A-10) 중화펩타이드

sc-373746 P
100 µg/0.5 ml
$68.00

Hello, I have EGFR(A-10): sc373746 antibody and p-EGFR (9H2): sc-57545, I would like to ask dilution for both and secondary reagent please . 

Asked by: 143KB
Thank you for your question. For Western Blot we recommend a starting dilution 1:100. The dilution range is 1:100-1:1000. More recommendations are given in the datasheet, which can be downloaded from the product page.
Answered by: Tech Support Europe
Date published: 2024-01-16

EGFR(A-10): sc-373746  Is the amino acid number of the epitope 1114-1147 counted from first Methionine or after cleavage of the signal peptide?

Asked by: Sakuchan
Thank you for your question. EGFR Antibody (A-10): sc-373746 is specific for an epitope mapping between amino acids 1114-1147 within a C-terminal cytoplasmic domain of EGFR of human origin. If you have a further question, please contact the local distributor or technical service.
Answered by: Sen Li
Date published: 2024-01-16

Can EGFR antibody A-10 sc-373746 be used on slide-fixed tumor cells (not on paraffin biopsies)?

Asked by: Anonymous
Thank you for your question. It would be helpful if you could call us, allowing for a more interactive discussion of this and other related questions.
Answered by: Technical Support
Date published: 2024-01-16

Does this ab recognize the mouse EGFR in tissus (IF), what is the dilution recommended? Thanks

Asked by: Any21
Thank you for your question. Yes, this antibody is recommended for IF/IHC on mouse tissue samples. We recommend a starting dilution of 1:50 for this antibody.
Answered by: Tech Service
Date published: 2024-04-17

Does this antibody against mutant forms of EGFR in mouse or human?

Asked by: Po Lee
Thank you for your question. EGFR Antibody (A-10): sc-373746 is specific for an epitope mapping between amino acids 1114-1147 within a C-terminal cytoplasmic domain of EGFR of human origin. EGFR proteins possessing this amino acid sequence should be recognized by this antibody without issue.
Answered by: Tech Support Europe
Date published: 2024-01-16

if I probe western blot with EGFR sc-373746, I don't have to against the blot with 2nd antibody. Is that right? Is this a phospho-EGFR antibody?

Asked by: Daisy II
This antibody is available unconjugated (sc-373476), in which case you must use a secondary anti-mouse IgG for detection. It is also available directly conjugated to HRP, and fluorescent probes, (sc-373476 HRP, etc.), in which case there is no secondary antibody needed for detection. This is not a phospho-specific EGFR antibody. It is reactive with total EGFR.
Answered by: Technical Support
Date published: 2024-04-17

HI, I need to do negative depletion of cells using an anti-EGF-receptor monoclonal antibody, mouse-human. can I use EGFR Antibody (A-10): sc-373746 for this purpose?

Asked by: Maria Vernon
Thank you for your question. We have not tested this antibody for any biological activity such as depletion, and so this is not a recommended application at this time. We do offer an alternative antibody which has been validated for use in biological studies, including neutralization, EGFR Antibody (R-1): sc-101, also provided azide-free for biological studies, sc-101 L.
Answered by: Technical Support
Date published: 2024-06-30

Is it positive for MCF-7 or MDA-MB-231 ?

Asked by: Gracess
Thank you for your question. We have published Western blot data on our website using this antibody in MDA-MB-231 whole cell lysates. We do not have any data in MCF-7.
Answered by: Technical Support
Date published: 2024-01-16
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Rated 5 out of 5 by from EGFR (A-10): sc-373746The specificity of the antibody is high, and the bands are clearly visible
Date published: 2023-05-15
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Rated 5 out of 5 by from very good antibodyI can detect band! The antibody was used for Western Blot in hepa1c1c7 cells, it works at a concentration of 1: 100 ~ 200.
Date published: 2022-12-07
Rated 4 out of 5 by from DetectableThis is a good antibody, we were able to detect in testing.
Date published: 2021-11-22
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EGFR 항체 (A-10) is rated 4.6 out of 5 by 66.
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