EGFL11 CRISPR/Cas9 KO Plasmid (h): sc-406815
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- EGFL11 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the EGFL11 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
EGFL11 CRISPR/Cas9 KO Plasmid (h) | sc-406815 | 20 µg | $397.00 |
Overview
EYS encodes EGFL11, a large extracellular matrix–associated protein enriched in photoreceptor cells where it contributes to the structural integrity and maintenance of the retinal outer segment. EGFL11 contains multiple EGF-like domains consistent with roles in protein–protein interactions, extracellular scaffolding, and organization of the periciliary environment that supports ciliary trafficking and phototransduction homeostasis. Disruption of EYS is strongly linked to inherited retinal degeneration, including autosomal recessive retinitis pigmentosa and related phenotypes characterized by progressive photoreceptor dysfunction. As a result, EYS/EGFL11 is frequently studied in pathways governing cilium-associated transport, extracellular matrix organization, and stress responses in retinal cells.
EGFL11 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the EYS gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the EYS together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the EYS open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish EGFL11 protein expression.
This CRISPR knockout system enables efficient generation of EYS-deficient cell models for investigation of EGFL11 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting EYS exon(s) critical for EGFL11 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple EYS genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by EGFL11 CRISPR/Cas9 KO Plasmid (h) and EGFL11 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the EYS locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by EGFL11 HDR Plasmid (h) and EGFL11 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by EYS homology arms to support homology-directed repair at defined EYS target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.