EFP Antibody (E-4): sc-166926

EFP Antibody (E-4) is a mouse monoclonal IgG2b kappa light chain antibody that detects EFP protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). Anti-EFP antibody (E-4) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. EFP protein, or estrogen-responsive finger protein, functions as a transcription factor that plays a crucial role in mediating estrogen signaling pathways. EFP protein is a member of the RING finger protein family, characterized by a zinc-binding domain known as the C3HC4 or RING finger motif, along with two B box domains and a coiled-coil region that serves as a transactivation domain. Regulation of EFP by estrogen is significant because EFP influences the growth, differentiation, and function of target cells across various tissues. EFP protein is involved in a signaling cascade where estrogen binds to its receptor, leading to upregulation of EFP gene expression, which activates transcription of secondary estrogen-responsive genes. Understanding EFP′s role in these pathways is essential for elucidating molecular mechanisms underlying estrogen′s effects on cellular processes and may provide insights into therapeutic targets for estrogen-related diseases.

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EFP Antibody (E-4) References:

1. Efp targets 14-3-3 sigma for proteolysis and promotes breast tumour growth.  |  Urano, T., et al. 2002. Nature. 417: 871-5. PMID: 12075357
2. A ubiquitin E3 ligase Efp is up-regulated by interferons and conjugated with ISG15.  |  Nakasato, N., et al. 2006. Biochem Biophys Res Commun. 351: 540-6. PMID: 17069755
3. Ligand-dependent transcription of estrogen receptor alpha is mediated by the ubiquitin ligase EFP.  |  Nakajima, A., et al. 2007. Biochem Biophys Res Commun. 357: 245-51. PMID: 17418098
4. Nuclear receptors enhance our understanding of transcription regulation.  |  Green, S. and Chambon, P. 1988. Trends Genet. 4: 309-14. PMID: 2853466
5. The steroid and thyroid hormone receptor superfamily.  |  Evans, RM. 1988. Science. 240: 889-95. PMID: 3283939
6. Molecular cloning, structure, and expression of mouse estrogen-responsive finger protein Efp. Co-localization with estrogen receptor mRNA in target organs.  |  Orimo, A., et al. 1995. J Biol Chem. 270: 24406-13. PMID: 7592654
7. The solution structure of the RING finger domain from the acute promyelocytic leukaemia proto-oncoprotein PML.  |  Borden, KL., et al. 1995. EMBO J. 14: 1532-41. PMID: 7729428
8. Genomic binding-site cloning reveals an estrogen-responsive gene that encodes a RING finger protein.  |  Inoue, S., et al. 1993. Proc Natl Acad Sci U S A. 90: 11117-21. PMID: 8248217
9. Multiple regulatory elements and binding proteins of the 5'-flanking region of the human estrogen-responsive finger protein (efp) gene.  |  Ikeda, K., et al. 1997. Biochem Biophys Res Commun. 236: 765-71. PMID: 9245730

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-EFP Antibody (E-4) has 8 citations in a variety of scientific publications.

Primary antibodies like Anti-EFP Antibody (E-4) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

EFP Antibody (E-4) Specifications

Alternate Product Name: EFP

Product Clone Name: E-4

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids: 158-173

Epitope Target: EFP

Epitope Species: human

Product Reactivity/Detection: EFP

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 8 citations in various scientific publications

Available Conjugates/Forms for EFP Antibody (E-4): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin, X: TransCruz Reagent