EF-Tu Antibody (A-5): sc-393924
- EF-Tu Antibody (A-5) is a mouse monoclonal IgG2a κ, cited in 6 publications, provided at 200 µg/ml
- raised against amino acids 171-455 mapping at the C-terminus of EF-Tu of human origin
- Anti-EF-Tu Antibody (A-5) is recommended for detection of EF-Tu of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-EF-Tu Antibody (A-5) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP and m-IgG2a BP-HRP are the preferred secondary detection reagents for EF-Tu Antibody (A-5) for WB applications. These reagents are now offered in bundles with EF-Tu Antibody (A-5) (see ordering information below).
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EF-Tu Antibody (A-5) is a mouse monoclonal IgG2a kappa light chain antibody that detects EF-Tu in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-EF-Tu antibody (A-5) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. EF-Tu, also known as mitochondrial precursor p43, is a crucial elongation factor that plays a vital role in protein biosynthesis on the ribosome. EF-Tu (A-5) antibody recognizes a protein responsible for fast and accurate delivery of aminoacyl-tRNAs to the translating ribosome, ensuring correct and efficient protein synthesis. EF-Tu′s function is particularly important because this protein not only facilitates amino acid incorporation into growing polypeptide chains but also protects the aminoester bond from hydrolysis until correct codon-anticodon pairing occurs. This mechanism maintains translation fidelity, which is critical for cellular function and overall organismal health. EF-Tu′s interaction with the ribosome is tightly regulated by GTP binding and hydrolysis, highlighting EF-Tu′s role as a multidomain GTPase integral to the translation process.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
EF-Tu Antibody (A-5) References:
- Possible evolution of factors involved in protein biosynthesis. | Nyborg, J. 1998. Acta Biochim Pol. 45: 883-94. PMID: 10397336
- Translational regulation by modifications of the elongation factor Tu. | Kraal, B., et al. 1999. Folia Microbiol (Praha). 44: 131-41. PMID: 10588048
- GTPases mechanisms and functions of translation factors on the ribosome. | Rodnina, MV., et al. 2000. Biol Chem. 381: 377-87. PMID: 10937868
- Domain III of elongation factor G from Thermus thermophilus is essential for induction of GTP hydrolysis on the ribosome. | Martemyanov, KA. and Gudkov, AT. 2000. J Biol Chem. 275: 35820-4. PMID: 10940297
- Visualization of elongation factor G on the Escherichia coli 70S ribosome: the mechanism of translocation. | Agrawal, RK., et al. 1998. Proc Natl Acad Sci U S A. 95: 6134-8. PMID: 9600930
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
EF-Tu Antibody (A-5) | sc-393924 | 200 µg/ml | $316.00 | |||
EF-Tu Antibody (A-5): m-IgG Fc BP-HRP Bundle | sc-526275 | 200 µg Ab; 10 µg BP | $354.00 | |||
EF-Tu Antibody (A-5): m-IgG2a BP-HRP Bundle | sc-546555 | 200 µg Ab; 10 µg BP | $354.00 | |||
EF-Tu Antibody (A-5) AC | sc-393924 AC | 500 µg/ml, 25% agarose | $416.00 | |||
EF-Tu Antibody (A-5) HRP | sc-393924 HRP | 200 µg/ml | $316.00 | |||
EF-Tu Antibody (A-5) FITC | sc-393924 FITC | 200 µg/ml | $330.00 | |||
EF-Tu Antibody (A-5) PE | sc-393924 PE | 200 µg/ml | $343.00 | |||
EF-Tu Antibody (A-5) Alexa Fluor® 488 | sc-393924 AF488 | 200 µg/ml | $357.00 | |||
EF-Tu Antibody (A-5) Alexa Fluor® 546 | sc-393924 AF546 | 200 µg/ml | $357.00 | |||
EF-Tu Antibody (A-5) Alexa Fluor® 594 | sc-393924 AF594 | 200 µg/ml | $357.00 | |||
EF-Tu Antibody (A-5) Alexa Fluor® 647 | sc-393924 AF647 | 200 µg/ml | $357.00 | |||
EF-Tu Antibody (A-5) Alexa Fluor® 680 | sc-393924 AF680 | 200 µg/ml | $357.00 | |||
EF-Tu Antibody (A-5) Alexa Fluor® 790 | sc-393924 AF790 | 200 µg/ml | $357.00 |