EF-1 γ Double Nickase Plasmid (h): sc-405691-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- EF-1 γ Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- EF-1 γ Double Nickase Plasmid (h) and EF-1 γ Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting EEF1G. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: EF-1 γ Antibody (C-7): sc-393378
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
EF-1 γ Double Nickase Plasmid (h) | sc-405691-NIC | 20 µg | $410.00 | |||
EF-1 γ Double Nickase Plasmid (h2) | sc-405691-NIC-2 | 20 µg | $410.00 |
EEF1G encodes eukaryotic translation elongation factor 1 gamma (EF-1γ), a core component of the eEF1 complex that delivers aminoacyl-tRNAs to the ribosome during mRNA translation. Beyond protein synthesis, EF-1γ contributes to cytoskeletal organization and proteostasis, linking translational control to cellular growth and stress adaptation programs. Altered expression or network connectivity of EEF1G has been observed in proliferative and stress-related cellular states, making it relevant for studying translation-associated remodeling in disease biology. As a highly connected node in RNA–protein and translation factor interactomes, EF-1γ is frequently used as a handle to interrogate coupling between protein synthesis, signaling, and cell-state transitions.
EF-1 γ Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the EEF1G locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within EEF1G. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt EEF1G function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of EEF1G-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.