Date published: 2026-4-13

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EF-1 γ Antibody (X5-P): sc-101035

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Datasheets
  • EF-1 γ Antibody (X5-P) is a mouse monoclonal IgG1 κ provided at 100 µg/ml
  • raised against recombinant EF-1 γ of human origin
  • recommended for detection of EF-1 γ of mouse, rat and human origin by WB, IP, IF and ELISA
  • At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for EF-1 γ Antibody (X5-P). This work is in progress.
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    EF-1 γ Antibody (X5-P) is a mouse monoclonal IgG1 antibody that detects EF-1 γ in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). EF-1 γ, also known as EEF1G or GIG35, is a crucial 437 amino acid subunit of the elongation factor-1 complex, which plays a vital role in protein synthesis by facilitating the delivery of aminoacyl-tRNAs to the ribosome. EF-1 γ is predominantly expressed in various tissues, including the stomach, pancreas, brain, lung, kidney, intestine, liver, and spleen, highlighting its importance in diverse biological processes. Notably, EF-1 γ contains an N-terminal glutathione transferase domain that anchors the complex to different cellular components, thereby influencing the assembly of multiprotein complexes that include aminoacyl-tRNA synthetases. Increased expression levels of EF-1 γ have been linked to pancreatic cancer, suggesting a potential role in oncogenic transformation. Anti-EF-1 γ antibody (X5-P) serves as an essential tool for researchers investigating the molecular mechanisms underlying protein synthesis and its implications in cancer biology.

    For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

    Ordering Information

    Product NameCatalog #UNITPriceQtyFAVORITES

    EF-1 γ Antibody (X5-P)

    sc-101035
    100 µg/ml
    $339.00

    I am using EF-1 γ (X5-P): sc-101035 for immunofluorescence, what dilution of this antibody should I use?

    Asked by: Dr Ninau Qelp
    Thank you for your question. We recommend a starting dilution of 1:50. However, optimal antibody concentration should be determined by titration. Please find our full immunofluorescence protocol here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
    Answered by: Technical Support
    Date published: 2017-03-24
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    Rated 5 out of 5 by from Good specificity and signal strength in WB in nonGood specificity and signal strength in WB in non-transfected 293T, human EF-1 transfected 293T and JAR whole cell lysates. -SCBT QC
    Date published: 2015-06-13
    Rated 5 out of 5 by from Produced exceptional immunofluorescence cytoplasmicProduced exceptional immunofluorescence cytoplasmic staining in paraformaldehyde-fixed HeLa cells. -SCBT QC
    Date published: 2015-05-16
    Rated 5 out of 5 by from Clean blot with single strong band at expectedClean blot with single strong band at expected size using mouse EF-1 transfected 293T and JAR whole cell lysates. -SCBT QC
    Date published: 2015-05-05
    Rated 5 out of 5 by from Good antibody for westernGood antibody for western, clean background and specific band in HL-60 whole cell lysate. - SCBT QC
    Date published: 2015-04-30
    Rated 5 out of 5 by from GreatGreat, strong band observed in WB in K-562 whole cell lysate. -SCBT QC
    Date published: 2015-04-24
    Rated 5 out of 5 by from Band detected at expected mw in 293 wholeBand detected at expected mw in 293 whole cell lysate. -SCBT QC
    Date published: 2015-04-02
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    EF-1 γ Antibody (X5-P) is rated 5.0 out of 5 by 6.
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