Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
EDG-2 CRISPR/Cas9 KO Plasmid (m): sc-420644
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- EDG-2 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the EDG-2 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: EDG-2 Antibody (B-10): sc-515665
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
EDG-2 CRISPR/Cas9 KO Plasmid (m) | sc-420644 | 20 µg | $397.00 |
Overview
Lpar1 encodes EDG-2, a G protein–coupled receptor for lysophosphatidic acid (LPA) that transduces extracellular lipid signals into intracellular programs controlling cell migration, proliferation, survival, and cytoskeletal remodeling. EDG-2 primarily engages Gαi/o, Gαq/11, and Gα12/13 pathways to activate PI3K–AKT, PLC–Ca2+ signaling, and RhoA/ROCK-mediated actin dynamics, influencing adhesion and contractility. In mouse tissues, Lpar1 signaling contributes to neurodevelopmental processes, vascular and fibroblast responses, and immune cell trafficking through regulation of chemotaxis and barrier function. Dysregulated LPA–EDG-2 activity has been implicated in inflammatory and fibrotic remodeling and in mechanisms relevant to tumor cell motility and microenvironmental crosstalk, supporting its utility in pathway-centric disease models.
EDG-2 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Lpar1 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Lpar1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Lpar1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish EDG-2 protein expression.
This CRISPR knockout system enables efficient generation of Lpar1-deficient cell models for investigation of EDG-2 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Lpar1 exon(s) critical for EDG-2 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Lpar1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by EDG-2 CRISPR/Cas9 KO Plasmid (m) and EDG-2 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Lpar1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by EDG-2 HDR Plasmid (m) and EDG-2 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Lpar1 homology arms to support homology-directed repair at defined Lpar1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.