EDD Antibody (C-3): sc-515485
- EDD Antibody (C-3) is a mouse monoclonal IgG1 κ provided at 200 µg/ml
- specific for an epitope mapping between amino acids 2-25 at the N-terminus of EDD of human origin
- recommended for detection of EDD of mouse, rat and human origin by WB, IP, IF and ELISA
- At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for EDD Antibody (C-3). This work is in progress.
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EDD Antibody (C-3) is a mouse monoclonal IgG1 antibody that detects EDD in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). EDD, or E3 identified by Differential Display, plays a crucial role in cellular regulation as EDD is involved in the ubiquitin-proteasome pathway, which is essential for protein degradation and turnover. This process is vital for maintaining cellular homeostasis, regulating various cellular functions, and controlling the cell cycle, thereby preventing uncontrolled cell growth that can lead to cancer. EDD contains a HECT (homologous to the E6-AP carboxyl terminus) domain, which is characteristic of E3 ubiquitin ligases, allowing EDD to facilitate the transfer of ubiquitin to target proteins. This post-translational modification is significant as ubiquitin tags proteins for degradation, thus influencing numerous cellular processes, including signal transduction, DNA repair, and apoptosis. Anti-EDD antibody (C-3) is an invaluable tool for researchers studying the mechanisms of protein regulation and the implications of EDD in cancer biology.
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EDD Antibody (C-3) References:
- EDD, the human hyperplastic discs protein, has a role in progesterone receptor coactivation and potential involvement in DNA damage response. | Henderson, MJ., et al. 2002. J Biol Chem. 277: 26468-78. PMID: 12011095
- EDD, the human orthologue of the hyperplastic discs tumour suppressor gene, is amplified and overexpressed in cancer. | Clancy, JL., et al. 2003. Oncogene. 22: 5070-81. PMID: 12902990
- The E3 ubiquitin ligase EDD regulates S-phase and G(2)/M DNA damage checkpoints. | Munoz, MA., et al. 2007. Cell Cycle. 6: 3070-7. PMID: 18073532
- Putative tumor suppressor EDD interacts with and up-regulates APC. | Ohshima, R., et al. 2007. Genes Cells. 12: 1339-45. PMID: 18076571
- alpha4 phosphoprotein interacts with EDD E3 ubiquitin ligase and poly(A)-binding protein. | McDonald, WJ., et al. 2010. J Cell Biochem. 110: 1123-9. PMID: 20544796
- Dyrk2-associated EDD-DDB1-VprBP E3 ligase inhibits telomerase by TERT degradation. | Jung, HY., et al. 2013. J Biol Chem. 288: 7252-62. PMID: 23362280
- HIV-1 Vpr protein inhibits telomerase activity via the EDD-DDB1-VPRBP E3 ligase complex. | Wang, X., et al. 2013. J Biol Chem. 288: 15474-80. PMID: 23612978
- Progestin-inducible EDD E3 ubiquitin ligase binds to α4 phosphoprotein to regulate ubiquitination and degradation of protein phosphatase PP2Ac. | McDonald, WJ., et al. 2014. Mol Cell Endocrinol. 382: 254-261. PMID: 24145130
- A functional genomic approach reveals the transcriptional role of EDD in the expression and function of angiogenesis regulator ACVRL1. | Chen, HW., et al. 2013. Biochim Biophys Acta. 1829: 1309-19. PMID: 24189493
- EDD, a ubiquitin-protein ligase of the N-end rule pathway, associates with spindle assembly checkpoint components and regulates the mitotic response to nocodazole. | Scialpi, F., et al. 2015. J Biol Chem. 290: 12585-94. PMID: 25833949
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
EDD Antibody (C-3) | sc-515485 | 200 µg/ml | $322.00 | |||
EDD (C-3) Neutralizing Peptide | sc-515485 P | 100 µg/0.5 ml | $69.00 |