Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Ect2 Double Nickase Plasmid (h): sc-401538-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Ect2 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Ect2 Double Nickase Plasmid (h) and Ect2 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting ECT2. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Ect2 Antibody (G-4): sc-514750
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Ect2 Double Nickase Plasmid (h) | sc-401538-NIC | 20 µg | $410.00 | |||
Ect2 Double Nickase Plasmid (h2) | sc-401538-NIC-2 | 20 µg | $410.00 |
ECT2 encodes epithelial cell transforming 2 (Ect2), a Rho guanine nucleotide exchange factor that activates RhoA, Rac1, and Cdc42 to coordinate cytoskeletal remodeling, cell polarity, and cytokinesis. Ect2 is regulated by phosphorylation and spatial sequestration, with mitotic relocalization enabling RhoA-driven contractile ring assembly and cleavage furrow ingression. Through Rho GTPase signaling and cross-talk with cell-cycle control networks, Ect2 influences adhesion dynamics, migration, and genome stability. Dysregulated ECT2 expression or activity has been linked to aberrant proliferation and invasive phenotypes in multiple tumor contexts, making it a useful node for studying oncogenic signaling and mitotic fidelity.
Ect2 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the ECT2 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within ECT2. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt ECT2 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of ECT2-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.