ECH1 Antibody (B-3): sc-515270

ECH1 Antibody (B-3) is a mouse monoclonal IgG1 kappa light chain antibody that detects ECH1 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). ECH1 Antibody (B-3) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. ECH1, or enoyl Coenzyme A hydratase 1, is a 328 amino acid protein that plays a crucial role in the fatty acid beta-oxidation pathway, specifically catalyzing the isomerization of 3-trans,5-cis-dienoyl-CoA to 2-trans,4-trans-dienoyl-CoA. This enzymatic function is vital for proper metabolism of fatty acids, which are essential for energy production and cellular function. ECH1 is localized in both the mitochondrion and peroxisome, organelles integral to lipid metabolism and energy homeostasis. The gene encoding ECH1 is located on human chromosome 19, a region that harbors several immunoglobulin superfamily members, including killer cell and leukocyte immunoglobulin-like receptors, various intercellular adhesion molecules, and Fc receptors, highlighting ECH1′s potential involvement in broader immunological processes.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

ECH1 Antibody (B-3) References:

1. Radiation hybrid mapping of three skeletal muscle genes (CKM, ECH1 and TNNT1) to porcine chromosome 6.  |  Davoli, R., et al. 2003. Anim Genet. 34: 302-3. PMID: 12873220
2. Overexpression of peroxisome proliferator-activated receptor-alpha (PPARalpha)-regulated genes in liver in the absence of peroxisome proliferation in mice deficient in both L- and D-forms of enoyl-CoA hydratase/dehydrogenase enzymes of peroxisomal beta-oxidation system.  |  Jia, Y., et al. 2003. J Biol Chem. 278: 47232-9. PMID: 14500732
3. Polymorphism of delta3,5-delta2,4-dienoyl-coenzyme A isomerase (the ECH1 gene product protein) in human striated muscle tissue.  |  Kovalyov, LI., et al. 2006. Biochemistry (Mosc). 71: 448-53. PMID: 16615866
4. Chronic quercetin exposure affects fatty acid catabolism in rat lung.  |  de Boer, VC., et al. 2006. Cell Mol Life Sci. 63: 2847-58. PMID: 17103110
5. Isolation and characterization of rat and human cDNAs encoding a novel putative peroxisomal enoyl-CoA hydratase.  |  FitzPatrick, DR., et al. 1995. Genomics. 27: 457-66. PMID: 7558027
6. Delta3,5-delta2,4-dienoyl-CoA isomerase from rat liver. Molecular characterization.  |  Filppula, SA., et al. 1998. J Biol Chem. 273: 349-55. PMID: 9417087

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-ECH1 Antibody (B-3) has 4 citations in a variety of scientific publications.

Primary antibodies like Anti-ECH1 Antibody (B-3) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

ECH1 Antibody (B-3) Specifications

Alternate Product Name: ECH1

Product Clone Name: B-3

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 47-65

Epitope Target: ECH1

Epitope Species: human

Product Reactivity/Detection: ECH1

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 4 citations in various scientific publications

Available Conjugates/Forms for ECH1 Antibody (B-3): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin