Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Ebi3 Double Nickase Plasmid (h): sc-401552-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Ebi3 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Ebi3 Double Nickase Plasmid (h) and Ebi3 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting EBI3. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Ebi3 Antibody (G-4): sc-166158
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Ebi3 Double Nickase Plasmid (h) | sc-401552-NIC | 20 µg | $410.00 |
EBI3 encodes the Epstein–Barr virus induced gene 3 (Ebi3), a secreted cytokine subunit that heterodimerizes with p28 to form IL-27 and with p35 to form IL-35, linking innate and adaptive immune regulation. Through IL-27/IL-35 signaling, Ebi3 influences JAK/STAT-mediated transcriptional programs that shape T cell differentiation, inflammatory cytokine production, and antigen-presenting cell function. Altered EBI3 expression has been associated with dysregulated immune responses in autoimmune and inflammatory settings and is frequently studied in the context of tumor immunology and chronic infection. These properties make EBI3 a useful target for dissecting cytokine circuitry and immunoregulatory pathways in human cell models.
Ebi3 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the EBI3 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within EBI3. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt EBI3 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of EBI3-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.