EBF4 CRISPR/Cas9 KO Plasmid (h): sc-402438
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- EBF4 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the EBF4 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
EBF4 CRISPR/Cas9 KO Plasmid (h) | sc-402438 | 20 µg | $397.00 |
Overview
EBF4 (early B-cell factor 4) is a helix–loop–helix transcription factor in the EBF family that binds DNA to regulate gene expression programs linked to cell fate specification and tissue differentiation. By coordinating transcriptional networks that intersect with chromatin regulation and developmental signaling, EBF4 can influence lineage-associated processes such as neuronal and immune-related gene expression. Altered control of EBF-family transcriptional circuits has been investigated in contexts including neurodevelopmental phenotypes and cancer-associated transcriptional reprogramming, making EBF4 a useful node for studying dysregulated differentiation. Functional interrogation of EBF4 supports mechanistic analysis of downstream targets, promoter/enhancer occupancy, and pathway-level effects on proliferation and identity.
EBF4 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the EBF4 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the EBF4 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the EBF4 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish EBF4 protein expression.
This CRISPR knockout system enables efficient generation of EBF4-deficient cell models for investigation of EBF4 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting EBF4 exon(s) critical for EBF4 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple EBF4 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by EBF4 CRISPR/Cas9 KO Plasmid (h) and EBF4 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the EBF4 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by EBF4 HDR Plasmid (h) and EBF4 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by EBF4 homology arms to support homology-directed repair at defined EBF4 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.