EBF Antibody (H-6): sc-398173
- EBF Antibody (H-6) is a mouse monoclonal IgG1 κ, cited in 1 publications, provided at 200 µg/ml
- specific for an epitope mapping between amino acids 376-405 within an internal region of EBF of human origin
- recommended for detection of EBF1 and EBF3 of mouse, rat, human and avian origin by WB, IP, IF and ELISA; also reactive with additional species, including and equine, canine, bovine, porcine and avian
- See EBF (C-8): sc-137065 for EBF antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
- m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for EBF Antibody (H-6) for WB applications. These reagents are now offered in bundles with EBF Antibody (H-6) (see ordering information below).
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EBF Antibody (H-6) is a mouse monoclonal IgG1 antibody that detects early B cell factor (EBF) in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). EBF is a crucial transcription factor that plays a significant role in the early stages of B lymphocyte maturation, orchestrating the expression of genes necessary for B cell development and function. Anti-EBF antibody (H-6) targets a protein primarily located in the nucleus, where EBF binds to specific DNA sequences to regulate the transcription of target genes, including those involved in B cell receptor signaling and immunoglobulin light chain gene recombination. Nuclear localization of EBF is vital, as this positioning allows for precise control over gene expression during B cell differentiation, ensuring that the immune system can effectively respond to pathogens. EBF monoclonal antibody (H-6) is an essential tool for researchers studying B cell biology and the mechanisms underlying immune responses, providing insights into the transcriptional regulation that governs B cell lineage commitment and function.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
EBF Antibody (H-6) References:
- Coordinate regulation of B cell differentiation by the transcription factors EBF and E2A. | O'Riordan, M. and Grosschedl, R. 1999. Immunity. 11: 21-31. PMID: 10435576
- The human V-preB promoter is a target for coordinated activation by early B cell factor and E47. | Gisler, R. and Sigvardsson, M. 2002. J Immunol. 168: 5130-8. PMID: 11994467
- Early B-cell factor (O/E-1) is a promoter of adipogenesis and involved in control of genes important for terminal adipocyte differentiation. | Akerblad, P., et al. 2002. Mol Cell Biol. 22: 8015-25. PMID: 12391167
- Roles of EBF and Pax-5 in B lineage commitment and development. | Maier, H. and Hagman, J. 2002. Semin Immunol. 14: 415-22. PMID: 12457614
- The Cxcl12, periostin, and Ccl9 genes are direct targets for early B-cell factor in OP-9 stroma cells. | Lagergren, A., et al. 2007. J Biol Chem. 282: 14454-62. PMID: 17374609
- IL-7 specifies B cell fate at the common lymphoid progenitor to pre-proB transition stage by maintaining early B cell factor expression. | Kikuchi, K., et al. 2008. J Immunol. 181: 383-92. PMID: 18566404
- Early B cell factor 2 regulates hematopoietic stem cell homeostasis in a cell-nonautonomous manner. | Kieslinger, M., et al. 2010. Cell Stem Cell. 7: 496-507. PMID: 20887955
- Restored immunosuppressive effect of mesenchymal stem cells on B cells after olfactory 1/early B cell factor-associated zinc-finger protein down-regulation in patients with systemic lupus erythematosus. | Feng, X., et al. 2014. Arthritis Rheumatol. 66: 3413-23. PMID: 25219468
- Cloning and functional characterization of early B-cell factor, a regulator of lymphocyte-specific gene expression. | Hagman, J., et al. 1993. Genes Dev. 7: 760-73. PMID: 8491377
- Early B-cell factor (EBF) down-regulates immunoglobulin heavy chain intron enhancer function in a plasmacytoma cell line. | Akerblad, P., et al. 1996. Scand J Immunol. 44: 89-92. PMID: 8693297
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
EBF Antibody (H-6) | sc-398173 | 200 µg/ml | $316.00 | |||
EBF Antibody (H-6): m-IgG Fc BP-HRP Bundle | sc-540921 | 200 µg Ab; 10 µg BP | $354.00 | |||
EBF Antibody (H-6): m-IgG1 BP-HRP Bundle | sc-542464 | 200 µg Ab; 20 µg BP | $354.00 | |||
EBF (H-6) Neutralizing Peptide | sc-398173 P | 100 µg/0.5 ml | $68.00 |