EBF Antibody (D-8): sc-137039

EBF Antibody (D-8) is a mouse monoclonal IgG2a antibody that detects early B cell factor (EBF) in mouse, rat, and human samples through various applications including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). EBF plays a crucial role in the maturation of B lymphocytes, acting as a transcription factor that regulates the expression of genes essential for B cell development. Specifically, EBF is involved in the early stages of B cell differentiation, where EBF targets promoter elements for B lymphoid kinase (Blk) and genes encoding components of the early stage B cell receptors (BCR). This regulation is vital for initiating immunoglobulin light chain gene recombination and Src kinase signaling, both of which are necessary for proper B cell function and immune response. Structurally, EBF is a basic helix-loop-helix (bHLH) homodimeric transcription factor, consisting of two subunits that bind to a specific DNA sequence, CCCNNGGG, through a zinc-coordination motif in its DNA recognition domain. The ability of EBF to interact with these promoter elements not only underscores EBF′s importance in B cell maturation but also highlights EBF′s potential role in influencing the expression of neuron-specific genes, particularly those related to olfactory functions, due to the presence of EBF binding sites in their promoters. anti-EBF antibody (D-8) is therefore an invaluable tool for researchers studying B cell development and its implications in immunological responses.

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EBF Antibody (D-8) References:

1. Olf-1/early B cell factor is a regulator of glut4 gene expression in 3T3-L1 adipocytes.  |  Dowell, P. and Cooke, DW. 2002. J Biol Chem. 277: 1712-8. PMID: 11696544
2. Cloning and characterization of a promoter flanking the early B cell factor (EBF) gene indicates roles for E-proteins and autoregulation in the control of EBF expression.  |  Smith, EM., et al. 2002. J Immunol. 169: 261-70. PMID: 12077253
3. Early B cell factor promotes B lymphopoiesis with reduced interleukin 7 responsiveness in the absence of E2A.  |  Seet, CS., et al. 2004. J Exp Med. 199: 1689-700. PMID: 15210745
4. Early B cell factor cooperates with Runx1 and mediates epigenetic changes associated with mb-1 transcription.  |  Maier, H., et al. 2004. Nat Immunol. 5: 1069-77. PMID: 15361869
5. Early B-cell Factor gene association with multiple sclerosis in the Spanish population.  |  Martínez, A., et al. 2005. BMC Neurol. 5: 19. PMID: 16255771
6. Transcription factor EBF restricts alternative lineage options and promotes B cell fate commitment independently of Pax5.  |  Pongubala, JM., et al. 2008. Nat Immunol. 9: 203-15. PMID: 18176567
7. Early B-cell factor regulates the expression of Hemokinin-1 in the olfactory epithelium and differentiating B lymphocytes.  |  Tran, AH., et al. 2011. J Neuroimmunol. 232: 41-50. PMID: 20965576
8. Induction of Epstein-Barr Virus Oncoprotein LMP1 by Transcription Factors AP-2 and Early B Cell Factor.  |  Murata, T., et al. 2016. J Virol. 90: 3873-3889. PMID: 26819314
9. Early B Cell Factor Activity Controls Developmental and Adaptive Thermogenic Gene Programming in Adipocytes.  |  Angueira, AR., et al. 2020. Cell Rep. 30: 2869-2878.e4. PMID: 32130892
10. Purification of early-B-cell factor and characterization of its DNA-binding specificity.  |  Travis, A., et al. 1993. Mol Cell Biol. 13: 3392-400. PMID: 8497258

The preferred EBF antibody is EBF Antibody (C-8): sc-137065.