EBF Antibody (D-8) recommended for detection of EBF1, EBF2, EBF3 and EBF4 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA

EBF Antibody (D-8): sc-137039

EBF Antibody (D-8) is rated 5.0 out of 5 by 3.
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Datasheets

    • EBF Antibody (D-8) is a mouse monoclonal IgG2a (kappa light chain) provided at 200 µg/ml
    • raised against amino acids 1-300 mapping at the N-terminus of EBF of human origin
    • recommended for detection of EBF1, EBF2, EBF3 and EBF4 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • See EBF (C-8): sc-137065 for EBF antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.

Can EBF (D-8): sc-137039 monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Asked by: DefinitelyNotMatt
Thank you for your question. Yes, EBF (D-8): sc-137039 has been validated for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-03-01
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Rated 5 out of 5 by from Band detected at expected mw in A549 and IMR Band detected at expected mw in A549 and IMR-32 whole cell lysates. -SCBT QC
Date published: 2015-04-10
Rated 5 out of 5 by from Good specificity and signal strength in human Good specificity and signal strength in human EBF1 transfected 293T whole cell lysate and Ramos nuclear extract. -SCBT QC
Date published: 2015-03-31
Rated 5 out of 5 by from Produced beautiful immunoperoxidase cytoplasmic Produced beautiful immunoperoxidase cytoplasmic staining in formalin fixed, paraffin-embedded human bone marrow tissue. -SCBT QC
Date published: 2015-03-22
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