Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
EAR2 CRISPR/Cas9 KO Plasmid (h): sc-404509
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- EAR2 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the EAR2 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
EAR2 CRISPR/Cas9 KO Plasmid (h) | sc-404509 | 20 µg | $397.00 |
Overview
NR2F6 encodes the orphan nuclear receptor EAR2, a sequence-specific transcription factor that modulates gene expression programs in a context-dependent manner. EAR2 participates in nuclear receptor signaling networks that intersect with chromatin regulation, cellular differentiation, and homeostatic control of immune and inflammatory transcriptional responses. Through promoter and enhancer binding, NR2F6 can tune pathways linked to T cell activation thresholds, cytokine signaling, and broader transcriptional repression/activation dynamics. Dysregulated NR2F6 activity has been associated with altered immune regulation and oncogenic transcriptional states, making it a useful node for mechanistic studies of gene regulatory circuitry in human cells.
EAR2 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the NR2F6 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the NR2F6 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the NR2F6 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish EAR2 protein expression.
This CRISPR knockout system enables efficient generation of NR2F6-deficient cell models for investigation of EAR2 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting NR2F6 exon(s) critical for EAR2 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple NR2F6 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by EAR2 CRISPR/Cas9 KO Plasmid (h) and EAR2 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the NR2F6 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by EAR2 HDR Plasmid (h) and EAR2 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by NR2F6 homology arms to support homology-directed repair at defined NR2F6 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.