Anti-EAAT4 Antibody (6D9) recommended for detection of EAAT4 of mouse, rat and human origin by WB, IP and ELISA

Anti-EAAT4 Antibody (6D9): sc-293344

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Datasheets

    • Anti-EAAT4 Antibody (6D9) is a mouse monoclonal IgG1 κ EAAT4 antibody, cited in 1 publications, provided at 100 µg/ml
    • raised against amino acids 500-564 of EAAT4 of human origin
    • recommended for detection of EAAT4 of mouse, rat and human origin by WB, IP and ELISA

Hi I tried this antibody sc-293344 (1:500 dilution) and got no signal in my cells (they express EAAT4 in qPCR). What is the size of this protein because in your photo it looks like its around 35kDa but I found in the literature it should be 62kDa. Thanks.

Asked by: smirkov
Thank you for your question. The expected molecular weight for EAAT4 is approximately 67 kDa. The Western blot data you are referring to on our website is not representative of the endogenous molecular weight for EAAT4 because it using a recombinant fusion protein (not full length). Please contact our Technical Service Department for assistance in troubleshooting this antibody.
Answered by: Technical Support
Date published: 2019-04-11

For Western Blot, is it recommended to use denatured or non-denatured conditions with EAAT4 (6D9): sc-293344 antibody?

Asked by: jerojero
Thank you for your question. We recommend this antibody for use in denatured Western Blot conditions. It has not been validated for use in non-denatured conditions. Please contact our Technical Service Department for further details or inquiries.
Answered by: Technical Support
Date published: 2017-03-27
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