E2F-8 Antibody (B-9): sc-514064
- E2F-8 Antibody (B-9) is a mouse monoclonal IgG1 λ E2F-8 antibody, cited in 3 publications, provided at 200 µg/ml
- raised against amino acids 664-764 mapping near the C-terminus of E2F-8 of human origin
- E2F-8 Antibody (B-9) is recommended for detection of E2F-8 of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-E2F-8 Antibody (B-9) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgGλ BP-HRP (mouse IgGλ binding protein-HRP) is the preferred secondary detection reagent for E2F-8 Antibody (B-9) for WB applications. This reagent is now offered in a bundle with E2F-8 Antibody (B-9) (see ordering information below). For additional m-IgGλ BP conjugates see our complete list of Mouse IgG Binding Proteins.
E2F-8 Antibody (B-9) is a mouse monoclonal IgG1 antibody that detects E2F-8 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). anti-E2F-8 antibody (B-9) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. E2F-8 is a crucial 867 amino acid nuclear protein that plays a significant role in regulating the cell cycle by functioning as a transcription factor. E2F-8 contains two DNA-binding domains and is part of a larger family of E2F transcription factors, which includes E2F-1 through E2F-7. E2F-8 can exist as a homodimer or form heterodimers with E2F-7, allowing binding to specific DNA sequences at the E2 recognition site, 5′-TTTC[CG]CGC-3′. This binding inhibits E2F-dependent transcription, thereby regulating the expression of genes essential for cell cycle progression. The gene encoding E2F-8 is located on human chromosome 11, a region associated with various genetic disorders, including Jervell and Lange-Nielsen syndrome and Jacobsen syndrome, highlighting E2F-8′s importance in cellular function and potential implications in human health.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
E2F-8 Antibody (B-9) References:
- E2F7, a novel E2F featuring DP-independent repression of a subset of E2F-regulated genes. | Di Stefano, L., et al. 2003. EMBO J. 22: 6289-98. PMID: 14633988
- Cloning and characterization of mouse E2F8, a novel mammalian E2F family member capable of blocking cellular proliferation. | Maiti, B., et al. 2005. J Biol Chem. 280: 18211-20. PMID: 15722552
- E2F-8: an E2F family member with a similar organization of DNA-binding domains to E2F-7. | Logan, N., et al. 2005. Oncogene. 24: 5000-4. PMID: 15897886
- Characterization of E2F8, a novel E2F-like cell-cycle regulated repressor of E2F-activated transcription. | Christensen, J., et al. 2005. Nucleic Acids Res. 33: 5458-70. PMID: 16179649
- Synergistic function of E2F7 and E2F8 is essential for cell survival and embryonic development. | Li, J., et al. 2008. Dev Cell. 14: 62-75. PMID: 18194653
- DNA-damage response control of E2F7 and E2F8. | Zalmas, LP., et al. 2008. EMBO Rep. 9: 252-9. PMID: 18202719
- E2f8 mediates tumor suppression in postnatal liver development. | Kent, LN., et al. 2016. J Clin Invest. 126: 2955-69. PMID: 27454291
- Cell cycle oscillators underlying orderly proteolysis of E2F8. | Wasserman, D., et al. 2020. Mol Biol Cell. 31: 725-740. PMID: 31995441
- E2F8 knockdown suppresses cell proliferation and induces cell cycle arrest via Wnt/β-Catenin pathway in ovarian cancer. | Zhang, M., et al. 2023. Chin J Physiol. 66: 266-275. PMID: 37635486
- E2F8 exerts cancer-promoting effects by transcriptionally activating RRM2 and E2F8 knockdown synergizes with WEE1 inhibition in suppressing lung adenocarcinoma. | Liu, K., et al. 2023. Biochem Pharmacol. 218: 115854. PMID: 37863324
- ENO1 promotes trophoblast invasion regulated by E2F8 in recurrent miscarriage. | Liu, Z., et al. 2024. FASEB J. 38: e23631. PMID: 38661062
- E2F8 facilitates malignant phenotypes of muscle-invasive bladder cancer via increasing MCM7 expression. | Liu, LY., et al. 2025. Biochem Cell Biol. 103: 1-14. PMID: 39601318
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
E2F-8 Antibody (B-9) | sc-514064 | 200 µg/ml | $316.00 | |||
E2F-8 Antibody (B-9): m-IgGλ BP-HRP Bundle | sc-524594 | 200 µg Ab, 40 µg BP | $354.00 | |||
E2F-8 Antibody (B-9) AC | sc-514064 AC | 500 µg/ml, 25% agarose | $416.00 | |||
E2F-8 Antibody (B-9) HRP | sc-514064 HRP | 200 µg/ml | $316.00 | |||
E2F-8 Antibody (B-9) FITC | sc-514064 FITC | 200 µg/ml | $330.00 | |||
E2F-8 Antibody (B-9) PE | sc-514064 PE | 200 µg/ml | $343.00 | |||
E2F-8 Antibody (B-9) Alexa Fluor® 488 | sc-514064 AF488 | 200 µg/ml | $357.00 | |||
E2F-8 Antibody (B-9) Alexa Fluor® 546 | sc-514064 AF546 | 200 µg/ml | $357.00 | |||
E2F-8 Antibody (B-9) Alexa Fluor® 594 | sc-514064 AF594 | 200 µg/ml | $357.00 | |||
E2F-8 Antibody (B-9) Alexa Fluor® 647 | sc-514064 AF647 | 200 µg/ml | $357.00 | |||
E2F-8 Antibody (B-9) Alexa Fluor® 680 | sc-514064 AF680 | 200 µg/ml | $357.00 | |||
E2F-8 Antibody (B-9) Alexa Fluor® 790 | sc-514064 AF790 | 200 µg/ml | $357.00 |