E2F-3 Antibody (PG30): sc-56665
- E2F-3 Antibody (PG30) is a mouse monoclonal IgG2a κ E2F-3 antibody, cited in 15 publications, provided at 200 µg/ml
- raised against recombinant E2F-3 of human origin
- E2F-3 Antibody (PG30) is recommended for detection of E2F-3 of mouse, rat and human origin by WB and IP; non cross-reactive with other members of the E2F family
- Anti-E2F-3 Antibody (PG30) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG2a BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for E2F-3 Antibody (PG30) for WB applications. These reagents are now offered in bundles with E2F-3 Antibody (PG30) (see ordering information below).
E2F-3 Antibody (PG30) is a mouse monoclonal IgG2a kappa light chain antibody that detects E2F-3 protein of mouse, rat, and human origin by western blotting (WB) and immunoprecipitation (IP). anti-E2F-3 antibody (PG30) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. E2F-3 protein plays a crucial role in regulating the cell cycle, particularly in the transition from G1 phase to S phase, where DNA synthesis occurs. This regulation is vital for proper cell proliferation and differentiation, as dysregulation of E2F-3 can lead to uncontrolled cell growth and is often implicated in cancer development. E2F-3 functions as a transcription factor that activates genes necessary for cell cycle progression, and E2F-3 activity is tightly controlled by interactions with retinoblastoma (Rb) protein. Rb protein, when functional, binds to E2F-3 and inhibits transcriptional activity, thereby preventing premature entry into S phase. Understanding E2F-3 dynamics and regulatory mechanisms is essential for developing therapeutic strategies targeting cell cycle dysregulation in various cancers.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
E2F-3 Antibody (PG30) References:
- A cDNA encoding a pRB-binding protein with properties of the transcription factor E2F. | Helin, K., et al. 1992. Cell. 70: 337-50. PMID: 1638634
- The E2F transcription factor is a cellular target for the RB protein. | Chellappan, SP., et al. 1991. Cell. 65: 1053-61. PMID: 1828392
- The T/E1A-binding domain of the retinoblastoma product can interact selectively with a sequence-specific DNA-binding protein. | Chittenden, T., et al. 1991. Cell. 65: 1073-82. PMID: 1828394
- The lncRNA NEAT1/miRNA-766-5p/E2F3 Regulatory Axis Promotes Prostate Cancer Progression. | Zhao, W., et al. 2022. J Oncol. 2022: 1866972. PMID: 35237319
- Circ_001422 aggravates osteosarcoma progression through targeting miR-497-5p/E2F3 axis. | Li, X., et al. 2023. J Biochem Mol Toxicol. 37: e23392. PMID: 37287369
- E2F3 accelerates the stemness of colon cancer cells by activating the STAT3 pathway. | Gao, Q., et al. 2023. Front Oncol. 13: 1203712. PMID: 37456248
- E2F-4, a new member of the E2F transcription factor family, interacts with p107. | Ginsberg, D., et al. 1994. Genes Dev. 8: 2665-79. PMID: 7958924
- E2F-4, a new member of the E2F gene family, has oncogenic activity and associates with p107 in vivo. | Beijersbergen, RL., et al. 1994. Genes Dev. 8: 2680-90. PMID: 7958925
- Binding to DNA and the retinoblastoma gene product promoted by complex formation of different E2F family members. | Krek, W., et al. 1993. Science. 262: 1557-60. PMID: 8248803
- Heterodimerization of the transcription factors E2F-1 and DP-1 leads to cooperative trans-activation. | Helin, K., et al. 1993. Genes Dev. 7: 1850-61. PMID: 8405995
- E2F-1 and E2F-3 are functionally distinct in their ability to promote myeloid cell cycle progression and block granulocyte differentiation. | Strom, DK., et al. 1998. Cell Growth Differ. 9: 59-69. PMID: 9438389
- E2F-6, a member of the E2F family that can behave as a transcriptional repressor. | Trimarchi, JM., et al. 1998. Proc Natl Acad Sci U S A. 95: 2850-5. PMID: 9501179
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
E2F-3 Antibody (PG30) | sc-56665 | 200 µg/ml | $322.00 | |||
E2F-3 Antibody (PG30): m-IgG Fc BP-HRP Bundle | sc-528617 | 200 µg Ab; 10 µg BP | $361.00 | |||
E2F-3 Antibody (PG30): m-IgGκ BP-HRP Bundle | sc-521040 | 200 µg Ab, 40 µg BP | $361.00 | |||
E2F-3 Antibody (PG30): m-IgG2a BP-HRP Bundle | sc-547130 | 200 µg Ab; 10 µg BP | $361.00 | |||
E2F-3 Antibody (PG30) AC | sc-56665 AC | 500 µg/ml, 25% agarose | $424.00 | |||
E2F-3 Antibody (PG30) HRP | sc-56665 HRP | 200 µg/ml | $322.00 | |||
E2F-3 Antibody (PG30) FITC | sc-56665 FITC | 200 µg/ml | $336.00 | |||
E2F-3 Antibody (PG30) PE | sc-56665 PE | 200 µg/ml | $349.00 | |||
E2F-3 Antibody (PG30) Alexa Fluor® 488 | sc-56665 AF488 | 200 µg/ml | $364.00 | |||
E2F-3 Antibody (PG30) Alexa Fluor® 546 | sc-56665 AF546 | 200 µg/ml | $364.00 | |||
E2F-3 Antibody (PG30) Alexa Fluor® 594 | sc-56665 AF594 | 200 µg/ml | $364.00 | |||
E2F-3 Antibody (PG30) Alexa Fluor® 647 | sc-56665 AF647 | 200 µg/ml | $364.00 | |||
E2F-3 Antibody (PG30) Alexa Fluor® 680 | sc-56665 AF680 | 200 µg/ml | $364.00 | |||
E2F-3 Antibody (PG30) Alexa Fluor® 790 | sc-56665 AF790 | 200 µg/ml | $364.00 |