E2F-3 Antibody (PG30): sc-56665

E2F-3 Antibody (PG30) is a mouse monoclonal IgG2a kappa light chain antibody that detects E2F-3 protein of mouse, rat, and human origin by western blotting (WB) and immunoprecipitation (IP). anti-E2F-3 antibody (PG30) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. E2F-3 protein plays a crucial role in regulating the cell cycle, particularly in the transition from G1 phase to S phase, where DNA synthesis occurs. This regulation is vital for proper cell proliferation and differentiation, as dysregulation of E2F-3 can lead to uncontrolled cell growth and is often implicated in cancer development. E2F-3 functions as a transcription factor that activates genes necessary for cell cycle progression, and E2F-3 activity is tightly controlled by interactions with retinoblastoma (Rb) protein. Rb protein, when functional, binds to E2F-3 and inhibits transcriptional activity, thereby preventing premature entry into S phase. Understanding E2F-3 dynamics and regulatory mechanisms is essential for developing therapeutic strategies targeting cell cycle dysregulation in various cancers.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

E2F-3 Antibody (PG30) References:

1. A cDNA encoding a pRB-binding protein with properties of the transcription factor E2F.  |  Helin, K., et al. 1992. Cell. 70: 337-50. PMID: 1638634
2. The E2F transcription factor is a cellular target for the RB protein.  |  Chellappan, SP., et al. 1991. Cell. 65: 1053-61. PMID: 1828392
3. The T/E1A-binding domain of the retinoblastoma product can interact selectively with a sequence-specific DNA-binding protein.  |  Chittenden, T., et al. 1991. Cell. 65: 1073-82. PMID: 1828394
4. The lncRNA NEAT1/miRNA-766-5p/E2F3 Regulatory Axis Promotes Prostate Cancer Progression.  |  Zhao, W., et al. 2022. J Oncol. 2022: 1866972. PMID: 35237319
5. Circ_001422 aggravates osteosarcoma progression through targeting miR-497-5p/E2F3 axis.  |  Li, X., et al. 2023. J Biochem Mol Toxicol. 37: e23392. PMID: 37287369
6. E2F3 accelerates the stemness of colon cancer cells by activating the STAT3 pathway.  |  Gao, Q., et al. 2023. Front Oncol. 13: 1203712. PMID: 37456248
7. E2F-4, a new member of the E2F transcription factor family, interacts with p107.  |  Ginsberg, D., et al. 1994. Genes Dev. 8: 2665-79. PMID: 7958924
8. E2F-4, a new member of the E2F gene family, has oncogenic activity and associates with p107 in vivo.  |  Beijersbergen, RL., et al. 1994. Genes Dev. 8: 2680-90. PMID: 7958925
9. Binding to DNA and the retinoblastoma gene product promoted by complex formation of different E2F family members.  |  Krek, W., et al. 1993. Science. 262: 1557-60. PMID: 8248803
10. Heterodimerization of the transcription factors E2F-1 and DP-1 leads to cooperative trans-activation.  |  Helin, K., et al. 1993. Genes Dev. 7: 1850-61. PMID: 8405995
11. E2F-1 and E2F-3 are functionally distinct in their ability to promote myeloid cell cycle progression and block granulocyte differentiation.  |  Strom, DK., et al. 1998. Cell Growth Differ. 9: 59-69. PMID: 9438389
12. E2F-6, a member of the E2F family that can behave as a transcriptional repressor.  |  Trimarchi, JM., et al. 1998. Proc Natl Acad Sci U S A. 95: 2850-5. PMID: 9501179

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-E2F-3 Antibody (PG30) has 15 citations in a variety of scientific publications.

Primary antibodies like Anti-E2F-3 Antibody (PG30) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

E2F-3 Antibody (PG30) Specifications

Alternate Product Name: E2F-3

Product Clone Name: PG30

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids:

Epitope Target: E2F-3

Epitope Species: human

Product Reactivity/Detection: E2F-3

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 15 citations in various scientific publications

Available Conjugates/Forms for E2F-3 Antibody (PG30): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin