E2F1 Antibody (E-2): sc-137060

E2F-1 is a mouse monoclonal IgG2b kappa light chain antibody designed to detect E2F-1 protein, also known as RBAP-1 or RBBP-3, across human, mouse, and rat species. Anti-E2F-1 antibody (E-2) targets a specific epitope within amino acids 301-437 of human E2F-1, making anti-E2F-1 antibody (E-2) suitable for applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). E2F-1 is a pivotal transcription factor that plays a crucial role in regulating the cell cycle, particularly the transition from G1 phase to S phase, by controlling the expression of genes necessary for DNA replication and cell proliferation. Within the cell, E2F-1 primarily resides in the nucleus, where E2F-1 interacts with retinoblastoma protein (Rb) to modulate activity. This interaction inhibits E2F-1′s transcriptional function, thereby preventing uncontrolled cell division. Additionally, E2F-1 forms heterodimers with DP-1 to facilitate transcription of genes involved in cell cycle progression and interacts with co-regulators such as p300, a histone acetyltransferase, and histone deacetylases (HDACs), which influence stability and transcriptional activity. These protein interactions are essential for maintaining proper cell cycle control and genomic integrity. Dysregulation of E2F-1, often through mutations or interactions with viral oncoproteins, can lead to unchecked cell proliferation and is a common feature in various cancers. Furthermore, E2F-1 is involved in apoptotic pathways, underscoring E2F-1′s role in balancing cell survival and programmed cell death. E2F-1 monoclonal antibody (E-2) is an invaluable tool for researchers investigating mechanisms of cell cycle regulation, tumorigenesis, and complex networks of protein interactions that govern cellular proliferation and apoptosis.

The preferred E2F1 antibody is E2F1 Antibody (KH95): sc-251.