Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
E-FABP Double Nickase Plasmid (h): sc-418529-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- E-FABP Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- E-FABP Double Nickase Plasmid (h) and E-FABP Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting FABP5. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: E-FABP Antibody (A-9): sc-365236
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
E-FABP Double Nickase Plasmid (h) | sc-418529-NIC | 20 µg | $410.00 | |||
E-FABP Double Nickase Plasmid (h2) | sc-418529-NIC-2 | 20 µg | $410.00 |
Fatty acid binding protein 5 (FABP5), also known as epidermal FABP (E-FABP), is a cytosolic lipid chaperone that binds long-chain fatty acids and other hydrophobic ligands to coordinate intracellular trafficking, storage, and utilization. By shaping lipid availability, E-FABP influences membrane remodeling, oxidative metabolism, and lipid-mediated signaling programs, including PPAR-regulated transcriptional networks. FABP5 activity has been linked to cellular differentiation and inflammatory responses, reflecting its roles in epidermal biology and immune cell function. Dysregulated FABP5 expression is frequently studied in the context of metabolic reprogramming and altered signaling states observed in cancer, skin disorders, and inflammatory disease models.
E-FABP Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the FABP5 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within FABP5. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt FABP5 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of FABP5-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.