SAM Transcription Activation: SAM system utilizing dCas9-VP64, MS2-P65-HSF1, and sgRNA for precise gene transcription activation.
E-cadherin Lentiviral Activation Particles (h): sc-400031-LAC
- Target species: human
- 200 µl of transduction-ready, high-titer CRISPR/dCas9 Lentiviral Activation Particles
- E-cadherin Lentiviral Activation Particles (h) is a synergistic activation mediator (SAM) transcription activation system designed to specifically and efficiently upregulate gene expression via lentiviral transduction of cells
- E-cadherin Lentiviral Activation Particles (h) contain the following SAM Activation elements: a deactivated Cas9 (dCas9) nuclease (D10A and N863A) fused to the transactivation domain VP64, an MS2-p65-HSF1 fusion protein and a target-specific 20 nt guide RNA. They also contain the blasticidin, hygromycin and puromycin resistance genes
- Upon transduction, the SAM complex binds to a site-specific region approximately 200-250 nt upstream of the transcriptional start site and provides robust recruitment of transcription factors for highly efficient gene activation
- gRNAs encoded by E-cadherin Lentiviral Activation Plasmid (h) and E-cadherin Lentiviral Activation Plasmid (h2) target distinct regulatory regions of the CDH1 promoter. One or both designs may be available
- Following transfection, gene activation efficiency can be assayed by WB, IF or IHC using antibody: E-cadherin Antibody (G-10): sc-8426
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
E-cadherin Lentiviral Activation Particles (h) | sc-400031-LAC | 200 µl | $455.00 |