dystrophin Antibody (MANDRA1): sc-73592

Dystrophin Antibody (MANDRA1) is a mouse monoclonal IgG1 kappa light chain antibody that detects dystrophin protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and immunohistochemistry. Anti-dystrophin antibody (MANDRA1) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Dystrophin plays a crucial role in the dystrophin-glycoprotein complex (DGC), which connects the F-actin cytoskeleton within muscle fibers to the extracellular matrix, thereby maintaining the structural integrity of muscle cells during contraction. This connection is vital for muscle function, as it helps to stabilize the muscle membrane and prevent damage during mechanical stress. A deficiency in dystrophin is linked to severe muscle disorders such as Duchenne (DMD) and Becker (BMD) muscular dystrophies, highlighting dystrophin′s importance in muscle health. The human dystrophin gene spans 2.4 megabases, contains over 80 exons, and produces a 14 kb mRNA that can undergo differential splicing to generate a variety of transcripts, each encoding different protein isoforms. Dystrophin constitutes approximately 0.002% of total striated muscle protein and is localized at triadic junctions in skeletal muscle, where dystrophin is believed to play a significant role in calcium ion homeostasis and force transmission, further underscoring dystrophin′s importance in muscle physiology.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

dystrophin Antibody (MANDRA1) References:

1. Dystrophin is a microtubule-associated protein.  |  Prins, KW., et al. 2009. J Cell Biol. 186: 363-9. PMID: 19651889
2. Skipping multiple exons of dystrophin transcripts using cocktail antisense oligonucleotides.  |  Echigoya, Y. and Yokota, T. 2014. Nucleic Acid Ther. 24: 57-68. PMID: 24380394
3. Dystrophin quantification: Biological and translational research implications.  |  Anthony, K., et al. 2014. Neurology. 83: 2062-9. PMID: 25355828
4. Dystrophin Analysis in Clinical Trials.  |  Aartsma-Rus, A. 2014. J Neuromuscul Dis. 1: 41-53. PMID: 27858668
5. Utrophin Compensates dystrophin Loss during Mouse Spermatogenesis.  |  Chen, HC., et al. 2017. Sci Rep. 7: 7372. PMID: 28785010
6. Dystrophin Dp71 Subisoforms Localize to the Mitochondria of Human Cells.  |  Niba, ETE., et al. 2021. Life (Basel). 11: PMID: 34575126
7. Dystrophin in the Neonatal and Adult Rat Intestine.  |  Lionarons, JM., et al. 2021. Life (Basel). 11: PMID: 34833031
8. The role of dystrophin isoforms and interactors in the brain.  |  Tetorou, K., et al. 2024. Brain.. PMID: 39673425
9. Dystrophin-associated proteins in muscular dystrophy.  |  Ozawa, E., et al. 1995. Hum Mol Genet. 4 Spec No: 1711-6. PMID: 8541869
10. Structure-function relationships in dystrophin and utrophin.  |  Winder, SJ. 1996. Biochem Soc Trans. 24: 497-501. PMID: 8736791

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-dystrophin Antibody (MANDRA1) has 10 citations in a variety of scientific publications.

Primary antibodies like Anti-dystrophin Antibody (MANDRA1) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

dystrophin Antibody (MANDRA1) Specifications

Alternate Product Name: dystrophin

Product Clone Name: MANDRA1

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 3200-3684

Epitope Target: dystrophin

Epitope Species: human

Product Reactivity/Detection: an epitope corresponding to amino acids 3558-3684 of dystrophin

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: fish

Applications: WB, IP, IF, IHC(P)

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 10 citations in various scientific publications

Available Conjugates/Forms for dystrophin Antibody (MANDRA1): AC: Agarose, AF488: Alexa Fluor^® 488, AF647: Alexa Fluor^® 647, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred dystrophin antibody is dystrophin Antibody (7A10): sc-47760.