Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Dyskerin Double Nickase Plasmid (h): sc-401278-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Dyskerin Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Dyskerin Double Nickase Plasmid (h) and Dyskerin Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting DKC1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Dyskerin Antibody (H-3): sc-373956
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Dyskerin Double Nickase Plasmid (h) | sc-401278-NIC | 20 µg | $410.00 | |||
Dyskerin Double Nickase Plasmid (h2) | sc-401278-NIC-2 | 20 µg | $410.00 |
DKC1 encodes dyskerin, an essential nucleolar protein that catalyzes pseudouridylation of rRNA and snRNA as part of H/ACA small nucleolar ribonucleoprotein complexes, supporting ribosome biogenesis and pre-mRNA splicing fidelity. Dyskerin also associates with the telomerase ribonucleoprotein complex to stabilize telomerase RNA and influence telomere maintenance, linking RNA modification to genome stability. Through these roles, DKC1 impacts translation control, nucleolar stress signaling, and cell proliferation programs. Genetic disruption or dysfunction of DKC1 is implicated in telomere biology disorders and ribosomopathies, providing a mechanistic bridge between impaired RNA processing and altered cellular homeostasis.
Dyskerin Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the DKC1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within DKC1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt DKC1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of DKC1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.