Dynein IC2, axonemal Antibody (X-7): sc-100932
- Dynein IC2, axonemal Antibody (X-7) is a mouse monoclonal IgG2a κ Dynein IC2, axonemal antibody provided at 100 µg/ml
- raised against recombinant Dynein IC2 axonemal of human origin
- recommended for detection of Dynein IC2, axonemal of human origin by WB, IP, IF and ELISA
- See Dynein IC1/2, cytosolic (74-1): sc-13524 for Dynein IC2, axonemal antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
- At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for Dynein IC2, axonemal Antibody (X-7). This work is in progress.
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Dynein IC2, axonemal Antibody (X-7) is a mouse monoclonal IgG2a kappa light chain antibody that detects Dynein IC2, axonemal (DNAI2) protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-DNAI2 antibody (X-7) is available as the non-conjugated form. Dyneins are essential multisubunit, high molecular weight ATPases that play a critical role in cellular transport by interacting with microtubules to convert the chemical energy of ATP into mechanical energy, facilitating movement. DNAI2 monoclonal antibody (X-7) recognizes a protein particularly important in cilia and flagella, where DNAI2 is involved in the sliding of microtubules within the axonemes, a process crucial for motility of these structures. Such motility is vital for various physiological processes, including sperm movement and mucus clearance in the respiratory tract. DNAI2 (X-7) antibody targets a component of a larger complex that includes heavy, intermediate, and light chains, which together ensure effective transport of cellular cargo towards the cell′s center. The carboxy terminus of DNAI2 is highly conserved and plays a significant role in microtubule-dependent motility, while amino terminal regions exhibit more variability, allowing for functional diversity. DNAI2 activity is regulated by several proteins, including dynactin, LIS1, and NudEL (NudE-like), which are crucial for proper functioning and coordination within the cell.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Dynein IC2, axonemal Antibody (X-7) References:
- Cytoplasmic dynein functions as a gear in response to load. | Mallik, R., et al. 2004. Nature. 427: 649-52. PMID: 14961123
- Cytoplasmic dynein nucleates microtubules to organize them into radial arrays in vivo. | Malikov, V., et al. 2004. Mol Biol Cell. 15: 2742-9. PMID: 15047865
- The dynein heavy chain family. | Asai, DJ. and Wilkes, DE. 2004. J Eukaryot Microbiol. 51: 23-9. PMID: 15068262
- High speed sliding of axonemal microtubules produced by outer arm dynein. | Seetharam, RN. and Satir, P. 2005. Cell Motil Cytoskeleton. 60: 96-103. PMID: 15605357
- Role of cytoplasmic dynein in the axonal transport of microtubules and neurofilaments. | He, Y., et al. 2005. J Cell Biol. 168: 697-703. PMID: 15728192
- NudEL targets dynein to microtubule ends through LIS1. | Li, J., et al. 2005. Nat Cell Biol. 7: 686-90. PMID: 15965467
- Cytoplasmic dynein nomenclature. | Pfister, KK., et al. 2005. J Cell Biol. 171: 411-3. PMID: 16260502
- Dynein motility: four heads are better than two. | McGrath, JL. 2005. Curr Biol. 15: R970-2. PMID: 16332531
- Genetic analysis of the cytoplasmic dynein subunit families. | Pfister, KK., et al. 2006. PLoS Genet. 2: e1. PMID: 16440056
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Dynein IC2, axonemal Antibody (X-7) | sc-100932 | 100 µg/ml | $339.00 |