Dynein HC CRISPR/Cas9 KO Plasmid (h): sc-400919
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Dynein HC CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Dynein HC genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Dynein HC Antibody (C-5): sc-514579
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Dynein HC CRISPR/Cas9 KO Plasmid (h) | sc-400919 | 20 µg | $397.00 |
Overview
DYNC1H1 encodes the heavy chain of cytoplasmic dynein-1, a microtubule-based motor that converts ATP hydrolysis into minus-end–directed transport. Dynein HC is central to intracellular trafficking, positioning of organelles, endosome and lysosome dynamics, axonal transport, and assembly and orientation of the mitotic spindle through coordinated activity with dynactin and adaptor proteins. By shaping microtubule-dependent transport and cell division processes, DYNC1H1 influences neuronal maintenance, polarity, and proteostasis pathways. Genetic disruption or dysfunction of dynein heavy chain has been linked to neurodevelopmental and neuromuscular disease mechanisms, making it a key target for studying transport defects and cytoskeletal stress responses.
Dynein HC CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the DYNC1H1 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the DYNC1H1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the DYNC1H1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Dynein HC protein expression.
This CRISPR knockout system enables efficient generation of DYNC1H1-deficient cell models for investigation of Dynein HC signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting DYNC1H1 exon(s) critical for Dynein HC function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple DYNC1H1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Dynein HC CRISPR/Cas9 KO Plasmid (h) and Dynein HC CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the DYNC1H1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Dynein HC HDR Plasmid (h) and Dynein HC HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by DYNC1H1 homology arms to support homology-directed repair at defined DYNC1H1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.