Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Dynamin II Double Nickase Plasmid (h): sc-401037-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Dynamin II Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Dynamin II Double Nickase Plasmid (h) and Dynamin II Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting DNM2. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Dynamin II Antibody (G-4): sc-166669
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Dynamin II Double Nickase Plasmid (h) | sc-401037-NIC | 20 µg | $410.00 | |||
Dynamin II Double Nickase Plasmid (h2) | sc-401037-NIC-2 | 20 µg | $410.00 |
DNM2 encodes Dynamin II, a large GTPase that catalyzes membrane fission during clathrin-mediated endocytosis and other vesicular trafficking events. Through interactions with phosphoinositides and SH3-domain proteins, Dynamin II coordinates receptor internalization, synaptic vesicle recycling, and endosome dynamics, linking membrane remodeling to actin cytoskeleton organization. DNM2 activity influences signaling pathways by controlling the trafficking and turnover of surface receptors, with downstream effects on cellular proliferation, migration, and homeostasis. Variants and dysregulation of DNM2 are associated with neuromuscular and neurodegenerative phenotypes and are widely studied in the context of membrane trafficking defects and cytoskeletal abnormalities.
Dynamin II Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the DNM2 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within DNM2. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt DNM2 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of DNM2-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.