Dynamin I Antibody (4E67): sc-70995

Dynamin I Antibody (4E67) is a mouse monoclonal IgG1 antibody that detects Dynamin I in mouse, rat, human, canine, and avian species through applications such as western blotting (WB), immunoprecipitation (IP), and immunofluorescence (IF). Dynamin I is a crucial GTPase involved in the process of endocytosis, which is essential for cellular communication and nutrient uptake. Dynamin I is predominantly localized in the central nervous system, where anti-Dynamin I antibody (4E67) plays a vital role in synaptic transmission and neurogenesis, processes that are fundamental for proper brain function and development. The structure of Dynamin I includes SH3 and proline-rich domains that facilitate interaction with various effector proteins, such as amphiphysin, which is highly expressed in the brain and is critical for the recruitment of Dynamin I to clathrin-coated pits. These interactions are necessary for the rapid endocytosis of synaptic vesicles, highlighting the importance of Dynamin I in maintaining synaptic integrity and plasticity. Dynamin I (4E67) antibody serves as an invaluable tool for researchers studying the dynamics of endocytosis and its implications in neurobiology.

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Dynamin I Antibody (4E67) References:

1. Phosphorylation of dynamin I on Ser-795 by protein kinase C blocks its association with phospholipids.  |  Powell, KA., et al. 2000. J Biol Chem. 275: 11610-7. PMID: 10766777
2. Characterization of the mouse dynamin I gene promoter and identification of sequences that direct expression in neuronal cells.  |  Yoo, J., et al. 2000. Biochem J. 351 Pt 3: 661-8. PMID: 11042120
3. Protein phosphorylation is required for endocytosis in nerve terminals: potential role for the dephosphins dynamin I and synaptojanin, but not AP180 or amphiphysin.  |  Cousin, MA., et al. 2001. J Neurochem. 76: 105-16. PMID: 11145983
4. Negative regulation of YY1 transcription factor on the dynamin I gene promoter.  |  Yoo, J., et al. 2001. Biochem Biophys Res Commun. 283: 340-3. PMID: 11327704
5. Chloride intracellular channel protein CLIC4 (p64H1) binds directly to brain dynamin I in a complex containing actin, tubulin and 14-3-3 isoforms.  |  Suginta, W., et al. 2001. Biochem J. 359: 55-64. PMID: 11563969
6. Interactions of phocein with nucleoside-diphosphate kinase, Eps15, and Dynamin I.  |  Baillat, G., et al. 2002. J Biol Chem. 277: 18961-6. PMID: 11872741
7. Myristyl trimethyl ammonium bromide and octadecyl trimethyl ammonium bromide are surface-active small molecule dynamin inhibitors that block endocytosis mediated by dynamin I or dynamin II.  |  Quan, A., et al. 2007. Mol Pharmacol. 72: 1425-39. PMID: 17702890
8. Differential phosphorylation of dynamin I isoforms in subcellular compartments demonstrates the hidden complexity of phosphoproteomes.  |  Chan, LS., et al. 2010. J Proteome Res. 9: 4028-37. PMID: 20560669
9. Phosphorylation and nitration of tyrosine residues affect functional properties of Synaptophysin and Dynamin I, two proteins involved in exo-endocytosis of synaptic vesicles.  |  Mallozzi, C., et al. 2013. Biochim Biophys Acta. 1833: 110-21. PMID: 23103755
10. Regulation of dynamin I GTPase activity by G protein betagamma subunits and phosphatidylinositol 4,5-bisphosphate.  |  Lin, HC. and Gilman, AG. 1996. J Biol Chem. 271: 27979-82. PMID: 8910402

The preferred Dynamin I antibody is Dynamin I Antibody (D5): sc-12724.