Dynactin p150 Antibody (12) recommended for detection of the p150 isoform of Dynactin 1 of mouse, rat, human and canine origin by WB, IP and IF

Dynactin p150 Antibody (12): sc-135890

Dynactin p150 Antibody (12) is rated 5.0 out of 5 by 2.
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Datasheets

    • Anti-Dynactin p150 Antibody (12) is a mouse monoclonal IgG1 (kappa light chain) Dynactin p150 antibody provided at 200 µg/ml
    • raised against amino acids 3-202 of Dynactin p150 of rat origin
    • recommended for detection of the p150 isoform of Dynactin 1 of mouse, rat, human and canine origin by WB, IP and IF
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.

I am using Dynactin p150 (12): sc-135890 for immunofluorescence, what dilution of this antibody should I use?

Asked by: DefinitelyNotMatt
Thank you for your question. We recommend a starting dilution of 1:50. However, optimal antibody concentration should be determined by titration. Please find our full immunofluorescence protocol here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2017-03-24
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Rated 5 out of 5 by from Positive results in western blot using human Positive results in western blot using human Dynactin 1 transfected 293 and HeLa whole cell lysates. -SCBT QC
Date published: 2015-07-14
Rated 5 out of 5 by from Robust band in western blot analysis of Dynactin Robust band in western blot analysis of Dynactin p150 expression in rat cerebrum tissue extract. -SCBT QC
Date published: 2014-08-17
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