Dvl-1/Dishevelled 1/DVL1 Double Nickase Plasmid (m): sc-420080-NIC
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Dvl-1/Dishevelled 1/DVL1 Double Nickase Plasmid (m) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Dvl-1/Dishevelled 1/DVL1 Double Nickase Plasmid (m) and Dvl-1/Dishevelled 1/DVL1 Double Nickase Plasmid (m2) encode distinct paired gRNA designs targeting Dvl1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Dvl-1/Dishevelled 1/DVL1 Antibody (3F12): sc-8025
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Dvl-1/Dishevelled 1/DVL1 Double Nickase Plasmid (m) | sc-420080-NIC | 20 µg | $410.00 |
Mouse Dvl1 encodes Dvl-1 (Dishevelled 1), a core cytoplasmic scaffold that transduces Wnt signals from Frizzled/LRP receptors into downstream responses. DVL1 coordinates canonical Wnt/β-catenin signaling as well as noncanonical planar cell polarity and Wnt/Ca²⁺ pathways by assembling multiprotein complexes that regulate receptor-proximal events and signalosome formation. Through these networks, DVL1 influences cell polarity, migration, cytoskeletal organization, and developmental patterning. Dysregulated Wnt–Dishevelled signaling is broadly relevant to studies of oncogenic signaling, neurodevelopmental phenotypes, and tissue homeostasis in mouse models.
Dvl-1/Dishevelled 1/DVL1 Double Nickase Plasmid (m) consists of a matched pair of plasmids engineered for high-specificity editing of the Dvl1 locus in mouse cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within Dvl1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt Dvl1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of Dvl1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.