dUTPase Antibody (H-9): sc-166856

dUTPase Antibody (H-9) is a mouse monoclonal IgG3 kappa light chain antibody that detects dUTPase protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). Anti-dUTPase antibody (H-9) is available as the non-conjugated form. dUTPase, also known as deoxyuridine 5′-triphosphate nucleotidohydrolase or DUT, plays a critical role in nucleotide metabolism and is essential for maintaining genomic integrity. DUT hydrolyzes dUTP to dUMP and diphosphate in the presence of magnesium ions, a reaction vital for preventing uracil incorporation into DNA. High levels of uracil in DNA can lead to mutations and ultimately cell death, highlighting DUT′s importance in cell viability and potential as a target for anticancer therapies. Additionally, dUMP, the product of this hydrolysis, serves as a precursor for thymidine nucleotides, crucial for DNA replication. DUT is expressed in various tissues, with isoforms DUT-N and DUT-M localizing to the nucleus and mitochondrion, respectively, with DUT-N being the predominant form. DUT′s ability to regulate nucleotide pools underscores its significance in cellular processes and relevance in cancer research.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

dUTPase Antibody (H-9) References:

1. Variations in patterns of DNA damage induced in human colorectal tumor cells by 5-fluorodeoxyuridine: implications for mechanisms of resistance and cytotoxicity.  |  Canman, CE., et al. 1992. Proc Natl Acad Sci U S A. 89: 10474-8. PMID: 1438236
2. Synthesis and high-throughput evaluation of triskelion uracil libraries for inhibition of human dUTPase and UNG2.  |  Jiang, YL., et al. 2006. Bioorg Med Chem. 14: 5666-72. PMID: 16678429
3. Structures of vaccinia virus dUTPase and its nucleotide complexes.  |  Samal, A., et al. 2007. Acta Crystallogr D Biol Crystallogr. 63: 571-80. PMID: 17452782
4. Kinetic mechanism of human dUTPase, an essential nucleotide pyrophosphatase enzyme.  |  Tóth, J., et al. 2007. J Biol Chem. 282: 33572-33582. PMID: 17848562
5. Active site closure facilitates juxtaposition of reactant atoms for initiation of catalysis by human dUTPase.  |  Varga, B., et al. 2007. FEBS Lett. 581: 4783-8. PMID: 17880943
6. Methylene substitution at the alpha-beta bridging position within the phosphate chain of dUDP profoundly perturbs ligand accommodation into the dUTPase active site.  |  Kovári, J., et al. 2008. Proteins. 71: 308-19. PMID: 17932923
7. Mutational analysis of the nucleotide binding site of Escherichia coli dCTP deaminase.  |  Thymark, M., et al. 2008. Arch Biochem Biophys. 470: 20-6. PMID: 17996716
8. Characterization of distinct nuclear and mitochondrial forms of human deoxyuridine triphosphate nucleotidohydrolase.  |  Ladner, RD., et al. 1996. J Biol Chem. 271: 7745-51. PMID: 8631816

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-dUTPase Antibody (H-9) has 1 citations in a variety of scientific publications.

Primary antibodies like Anti-dUTPase Antibody (H-9) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

dUTPase Antibody (H-9) Specifications

Alternate Product Name: dUTPase

Product Clone Name: H-9

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₃ kappa light chain

Amino Acids: 203-219

Epitope Target: dUTPase

Epitope Species: human

Product Reactivity/Detection: dUTPase

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: canine, bovine and porcine

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₃ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 1 citations in various scientific publications

Available Conjugates/Forms for dUTPase Antibody (H-9): P: Blocking Peptide