Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
DUSP8 Double Nickase Plasmid (h): sc-404385-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- DUSP8 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- DUSP8 Double Nickase Plasmid (h) and DUSP8 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting DUSP8. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: DUSP8 Antibody (B-9): sc-271250
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
DUSP8 Double Nickase Plasmid (h) | sc-404385-NIC | 20 µg | $410.00 | |||
DUSP8 Double Nickase Plasmid (h2) | sc-404385-NIC-2 | 20 µg | $410.00 |
Dual specificity phosphatase 8 (DUSP8), also known as M3/6, is a MAPK phosphatase that dephosphorylates threonine and tyrosine residues on activated MAPKs, with prominent activity toward JNK and p38 signaling. By constraining stress-activated kinase cascades, DUSP8 helps regulate cell proliferation, apoptosis, differentiation, and inflammatory transcriptional programs. DUSP8 is induced in response to cellular stress and growth factor cues, contributing to negative-feedback control of MAPK pathway dynamics. Dysregulated DUSP8 expression or signaling has been linked to altered MAPK/JNK pathway output in cancer biology, metabolic and inflammatory states, and neurological phenotypes, making it a useful node for pathway mechanism studies.
DUSP8 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the DUSP8 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within DUSP8. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt DUSP8 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of DUSP8-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.