Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
DUSP5 Double Nickase Plasmid (h): sc-401837-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- DUSP5 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- DUSP5 Double Nickase Plasmid (h) and DUSP5 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting DUSP5. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: DUSP5 Antibody (H-9): sc-393801
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
DUSP5 Double Nickase Plasmid (h) | sc-401837-NIC | 20 µg | $410.00 | |||
DUSP5 Double Nickase Plasmid (h2) | sc-401837-NIC-2 | 20 µg | $410.00 |
Dual specificity protein phosphatase 5 (DUSP5) is a nuclear MAPK phosphatase that preferentially dephosphorylates activated ERK1/2, acting as a feedback regulator of the RAF–MEK–ERK signaling cascade. By modulating ERK-dependent transcriptional programs, DUSP5 influences cell-cycle progression, differentiation, and stress-responsive gene expression. Altered DUSP5 expression or activity has been linked to dysregulated mitogenic signaling and pathway rewiring observed in multiple cancer contexts, and it is also studied in models of cardiovascular remodeling and inflammatory signaling. As a pathway-specific negative regulator, DUSP5 is frequently used to probe mechanisms controlling ERK signal amplitude, duration, and nuclear retention.
DUSP5 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the DUSP5 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within DUSP5. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt DUSP5 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of DUSP5-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.