DUS1L Antibody (H-12): sc-393586

DUS1L Antibody (H-12) is a mouse monoclonal IgM antibody that detects DUS1L protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). Anti-DUS1L antibody (H-12) is available as the non-conjugated form. DUS1L, also known as dihydrouridine synthase 1-like, tRNA-dihydrouridine synthase 1-like, DUS1, or PP3111, is a 473 amino acid protein that plays a crucial role in the modification of tRNA molecules by catalyzing the synthesis of dihydrouridine, a modified base found in the D-loop of many tRNAs. This modification is essential for proper folding and stability of tRNA, which in turn is vital for accurate protein synthesis and cellular function. DUS1L binds to flavin adenine dinucleotide (FAD) as a cofactor, and DUS1L gene is located on human chromosome 17, a region significant due to its association with key tumor suppressor genes such as p53 and BRCA1. p53 protein is critical for maintaining cellular genetic integrity, as p53 regulates cell fate decisions between DNA repair and apoptosis, while BRCA1 is recognized for its role in DNA repair and its association with hereditary breast and ovarian cancers. DUS1L′s importance in tRNA modification highlights its potential impact on gene expression and cellular health, making DUS1L a valuable target for research in cancer biology and other diseases.

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DUS1L Antibody (H-12) References:

1. p53 website and analysis of p53 gene mutations in human cancer: forging a link between epidemiology and carcinogenesis.  |  Soussi, T., et al. 2000. Hum Mutat. 15: 105-13. PMID: 10612830
2. Distinct pattern of p53 phosphorylation in human tumors.  |  Minamoto, T., et al. 2001. Oncogene. 20: 3341-7. PMID: 11423984
3. Three primary malignancies related to BRCA mutation successively occurring in a BRCA1 185delAG mutation carrier.  |  Piura, B., et al. 2001. Eur J Obstet Gynecol Reprod Biol. 97: 241-4. PMID: 11451557
4. A conserved family of Saccharomyces cerevisiae synthases effects dihydrouridine modification of tRNA.  |  Xing, F., et al. 2002. RNA. 8: 370-81. PMID: 12003496
5. Closing in on a breast cancer gene on chromosome 17q.  |  Hall, JM., et al. 1992. Am J Hum Genet. 50: 1235-42. PMID: 1598904
6. Cyanobacterial Catalase Activity Prevents Oxidative Stress Induced by Pseudomonas fluorescens DUS1-27 from Inhibiting Brassica napus L. (canola) Growth.  |  Hudek, L., et al. 2018. Microbes Environ. 33: 407-416. PMID: 30473566
7. Parallel-reaction monitoring revealed altered expression of a number of epitranscriptomic reader, writer, and eraser proteins accompanied with colorectal cancer metastasis.  |  Qi, TF., et al. 2023. Proteomics. 23: e2200059. PMID: 35443089
8. Human DUS1L catalyzes dihydrouridine modification at tRNA positions 16/17, and DUS1L overexpression perturbs translation.  |  Matsuura, J., et al. 2024. Commun Biol. 7: 1238. PMID: 39354220
9. A detailed study of loss of heterozygosity on chromosome 17 in tumours from Li-Fraumeni patients carrying a mutation to the TP53 gene.  |  Varley, JM., et al. 1997. Oncogene. 14: 865-71. PMID: 9047394
10. The Li-Fraumeni syndrome: an inherited susceptibility to cancer.  |  Evans, SC. and Lozano, G. 1997. Mol Med Today. 3: 390-5. PMID: 9302689
11. Loss of heterozygosity for defined regions on chromosomes 3, 11 and 17 in carcinomas of the uterine cervix.  |  Kersemaekers, AM., et al. 1998. Br J Cancer. 77: 192-200. PMID: 9460988

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-DUS1L Antibody (H-12) has 0 citations in a variety of scientific publications.

Primary antibodies like Anti-DUS1L Antibody (H-12) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

DUS1L Antibody (H-12) Specifications

Alternate Product Name: DUS1L

Product Clone Name: H-12

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgM kappa light chain

Amino Acids: 366-389

Epitope Target: DUS1L

Epitope Species: human

Product Reactivity/Detection: DUS1L

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: equine and canine

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgM kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 0 citations in various scientific publications

Available Conjugates/Forms for DUS1L Antibody (H-12): P: Blocking Peptide