Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
DUOX2 CRISPR/Cas9 KO Plasmid (h): sc-403534
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- DUOX2 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the DUOX2 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: DUOX2 Antibody (E-8): sc-398681
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
DUOX2 CRISPR/Cas9 KO Plasmid (h) | sc-403534 | 20 µg | $397.00 |
Overview
DUOX2 (dual oxidase 2) encodes a membrane-associated NADPH oxidase that generates hydrogen peroxide at the apical surface of epithelial cells, providing substrate for peroxidase-mediated reactions and redox-dependent signaling. As part of mucosal innate defense, DUOX2 activity is integrated with calcium signaling, cytokine-driven JAK/STAT responses, and oxidative stress pathways that shape barrier function and antimicrobial programs. Dysregulated DUOX2 expression or activity has been linked to inflammatory bowel disease susceptibility, chronic mucosal inflammation, and altered host–microbiome interactions, and it is also studied in the context of thyroid hormonogenesis and epithelial carcinogenesis. Because DUOX2-derived ROS can modulate MAPK and NF-κB signaling, it is frequently interrogated for effects on proliferation, differentiation, and DNA damage responses in epithelial models.
DUOX2 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the DUOX2 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the DUOX2 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the DUOX2 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish DUOX2 protein expression.
This CRISPR knockout system enables efficient generation of DUOX2-deficient cell models for investigation of DUOX2 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting DUOX2 exon(s) critical for DUOX2 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple DUOX2 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by DUOX2 CRISPR/Cas9 KO Plasmid (h) and DUOX2 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the DUOX2 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by DUOX2 HDR Plasmid (h) and DUOX2 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by DUOX2 homology arms to support homology-directed repair at defined DUOX2 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.