DUOX1 CRISPR/Cas9 KO Plasmid (m): sc-430265
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- DUOX1 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the DUOX1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
DUOX1 CRISPR/Cas9 KO Plasmid (m) | sc-430265 | 20 µg | $397.00 |
Overview
Duox1 encodes dual oxidase 1 (DUOX1), a NADPH oxidase family enzyme that generates hydrogen peroxide at the cell surface and within secretory pathways. In mouse epithelia and mucosal tissues, DUOX1-derived reactive oxygen species contribute to redox signaling, innate host defense, and regulation of processes such as wound repair and inflammatory responses. DUOX1 activity interfaces with calcium-dependent signaling and influences downstream pathways sensitive to oxidative cues, including MAPK and NF-κB-associated transcriptional programs. Dysregulated DUOX1-mediated ROS production has been implicated in models of airway inflammation, epithelial barrier dysfunction, and oxidative stress biology relevant to disease mechanisms.
DUOX1 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Duox1 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Duox1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Duox1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish DUOX1 protein expression.
This CRISPR knockout system enables efficient generation of Duox1-deficient cell models for investigation of DUOX1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Duox1 exon(s) critical for DUOX1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Duox1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by DUOX1 CRISPR/Cas9 KO Plasmid (m) and DUOX1 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Duox1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by DUOX1 HDR Plasmid (m) and DUOX1 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Duox1 homology arms to support homology-directed repair at defined Duox1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.